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Enhancing Lipid Production in the Arctic Microalga Chlamydomonas sp. KNM0029C by Using Statistical Optimization of Medium Components

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Title
Enhancing Lipid Production in the Arctic Microalga Chlamydomonas sp. KNM0029C by Using Statistical Optimization of Medium Components
Other Titles
배지 성분의 통계적 최적화를 이용한 북극 미세조류 Chlamydomonas sp. KNM0029C의 지질 생산 증대
Authors
Kim, Eun Jae
정웅식
Kim, Sanghee
Choi, Han-Gu
Han, Se Jong
Keywords
Arctic microalgae; Chlamydomonas sp. KNM0029C; Lipid; Response surface methodology; Statistical optimization; Tris-acetate-phosphate
Issue Date
2019
Citation
Kim, Eun Jae, et al. 2019. "Enhancing Lipid Production in the Arctic Microalga Chlamydomonas sp. KNM0029C by Using Statistical Optimization of Medium Components". KSBB Journal, 34(3): 173-177.
Abstract
A sequential statistical optimization was applied to the components of Tris-acetate-phosphate (TAP) medium, to enhance the cell density and lipid production of Arctic microalga Chlamydomonas sp. KNM0029C. The evaluation was preformed through an elimination process and Plackett-Burman design to select for significant factors, contributing toward cell growth. NH4Cl, MgSO4?7H2O, potassium phosphate, and trace elements were established as the effective components and used as variable factors in Box-Behnken design test, - a response surface methodology (RSM). The final optimized concentrations were 0.54, 0.16, 0.04, and 0.08 g/L of NH4Cl, MgSO4?7H2O, potassium phosphate, and trace elements, respectively. Overall, 9.9% enhanced lipid production was achieved by using optimized TAP medium at 4?C. The results of the present study could potentially contribute toward large-scale lipid production at low temperatures.
URI
http://repository.kopri.re.kr/handle/201206/10104
DOI
http://dx.doi.org/10.7841/ksbbj.2019.34.3.173
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