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De Novo Transcriptome Assembly and Comparative Analysis of Differentially Expressed Genes Involved in Cold Acclimation and Freezing Tolerance of the Arctic Moss Aulacomnium turgidum (Wahlenb.) Schwaegr

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dc.contributor.authorKang, Pilsung-
dc.contributor.authorYoo, Yo-Han-
dc.contributor.authorKim Dong-Il-
dc.contributor.authorYim, Joung Han-
dc.contributor.authorLee, Hyoungseok-
dc.date.accessioned2023-12-06T16:37:43Z-
dc.date.available2023-12-06T16:37:43Z-
dc.date.issued2023-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/14854-
dc.description.abstractCold acclimation refers to a phenomenon in which plants become more tolerant to freezing after exposure to non-lethal low temperatures. Aulacomnium turgidum (Wahlenb.) Schwaegr is a moss found in the Arctic that can be used to study the freezing tolerance of bryophytes. To improve our understanding of the cold acclimation effect on the freezing tolerance of A. turgidum, we compared the electrolyte leakage of protonema grown at 25 degrees C (non-acclimation; NA) and at 4 degrees C (cold acclimation; CA). Freezing damage was significantly lower in CA plants frozen at -12 degrees C (CA-12) than in NA plants frozen at -12 degrees C (NA-12). During recovery at 25 degrees C, CA-12 demonstrated a more rapid and greater level of the maximum photochemical efficiency of photosystem II than NA-12, indicating a greater recovery capacity for CA-12 compared to NA-12. For the comparative analysis of the transcriptome between NA-12 and CA-12, six cDNA libraries were constructed in triplicate, and RNA-seq reads were assembled into 45,796 unigenes. The differential gene expression analysis showed that a significant number of AP2 transcription factor genes and pentatricopeptide repeat protein-coding genes related to abiotic stress and the sugar metabolism pathway were upregulated in CA-12. Furthermore, starch and maltose concentrations increased in CA-12, suggesting that cold acclimation increases freezing tolerance and protects photosynthetic efficiency through the accumulation of starch and maltose in A. turgidum. A de novo assembled transcriptome can be used to explore genetic sources in non-model organisms.-
dc.languageEnglish-
dc.subject.classificationDasan Station-
dc.titleDe Novo Transcriptome Assembly and Comparative Analysis of Differentially Expressed Genes Involved in Cold Acclimation and Freezing Tolerance of the Arctic Moss Aulacomnium turgidum (Wahlenb.) Schwaegr-
dc.title.alternative북극 이끼 Aulacomnium turgidum의 저온 순응과 결빙 내성 관련된 유전자 발현 분석-
dc.typeArticle-
dc.identifier.bibliographicCitationKang, Pilsung, et al. 2023. "De Novo Transcriptome Assembly and Comparative Analysis of Differentially Expressed Genes Involved in Cold Acclimation and Freezing Tolerance of the Arctic Moss Aulacomnium turgidum (Wahlenb.) Schwaegr". <em>PLANTS-BASEL</em>, 12(6): 1-19.-
dc.citation.titlePLANTS-BASEL-
dc.citation.volume12-
dc.citation.number6-
dc.identifier.doi10.3390/plants12061250-
dc.citation.startPage1-
dc.citation.endPage19-
dc.description.articleClassificationSCIE-
dc.description.jcrRateJCR 2021:16.318-
dc.subject.keywordAulacomnium turgidum-
dc.subject.keywordRNA-seq-
dc.subject.keywordcold acclimation-
dc.subject.keywordde novo assembly-
dc.subject.keywordfreezing stress-
dc.identifier.localId2023-0042-
Appears in Collections  
2023-2023, Commercialization of new Biomaterials from polar organisms (23-23) / Kim, Il-Chan (PE23150)
2023-2023, Post-Polar Genomics Project: Functional genomic study for securing of polar useful genes (23-23) / Kim, Jin-Hyoung (PE23160)
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