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β-Naphthoflavone Induces Oxidative Stress inthe Intertidal Copepod, Tigriopus japonicus

Cited 7 time in scopus
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Title
β-Naphthoflavone Induces Oxidative Stress inthe Intertidal Copepod, Tigriopus japonicus
Authors
Rhee, Jae-Sung
Lee, Young-Mi
Kim, Bo-Mi
Leung, Kenneth Mei Yee
Kim, Il-Chan
Yim, Joung Han
Lee, Jae-Seong
Subject
Environmental Sciences & Ecology; Toxicology
Keywords
Tigriopus japonicus; Oxidative stress; Biomarkers; β-naphthoflavone; β-NF; Water Resources
Issue Date
2013
Citation
Rhee, Jae-Sung, et al. 2013. "β-Naphthoflavone Induces Oxidative Stress inthe Intertidal Copepod, Tigriopus japonicus". Environmental Toxicology, 30(3): 332-342.
Abstract
β-Naphtoflavone (b-NF) is a flavonoid and enhances oxidative stress in vertebrates with little information from aquatic invertebrates as yet. In this study, we investigated the effects of b-NF on the antioxidant defense systems of the intertidal copepod Tigriopus japonicus. To measure the β-NFtriggered changes in oxidative stress markers, such as intracellular reactive oxygen species (ROS), glutathione (GSH) concentration, residual glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR), and superoxide dismutase (SOD) activity, T. japonicus were exposed to b-NF (0.5 and 1 mg/L) for 72 h. Significant (P<0.05) induction of the intracellular ROS content (%) was observed in 1 mg/L of β-NF exposed T. japonicus, compared to the negative control and H2O2-exposed group. The GSH levels were significantly increased in the 0.5 mg/L of β-NF-exposed group for 12 h and 1 mg/L of β- NF-exposed groups for 12?24 h. GPx, GST, and GR activities showed a significant increase in the 1 mg/L b-NF-exposed group, indicating that b-NF induces oxidative stress in T. japonicus. To understand the effects of β-NF at the level of transcript expression, a 6K microarray analysis was employed. Transcript profiles of selected antioxidant-related genes were modulated after 72 h exposure to 1 mg/L of β-NF. From microarray data, 10 GST isoforms, GR, GPx, PH-GPx, and Se-GPx were chosen for a time-course test by real-time RT-PCR. T. japonicus GST-S, GST-O, GST-M, and GST-D1 were significantly increased in a 1 mg/L b-NF-exposed group. T. japonicus GPx, GR, and Se-GPx mRNA levels were also significantly increased at both concentrations. Our results revealed that oxidative stress was induced by β-NF exposure in T. japonicus.
DOI
http://dx.doi.org/10.1002/tox.21911
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