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Cryoprotective properties and preliminary characterization of exopolysaccharide (P-Arcpo 15) produced by the Arctic bacterium Pseudoalteromonas elyakovii Arcpo 15

Cited 14 time in wos
Cited 15 time in scopus

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DC Field Value Language
dc.contributor.authorKim, Sung Jin-
dc.contributor.authorKim, Byung-Gee-
dc.contributor.authorPark, Ha Ju-
dc.contributor.authorYim, Joung Han-
dc.coverage.spatialChukchi Sea-
dc.date.accessioned2017-08-03T12:52:50Z-
dc.date.available2017-08-03T12:52:50Z-
dc.date.issued2016-
dc.description.abstractTwenty-two bacterial strains that secrete exopolysaccharides (EPS) were isolated from marine samples obtained from the Chukchi Sea in the Arctic Ocean; of these, seven strains were found to be capable of producing cryoprotective EPS. The ArcPo 15 strain was isolated based on its ability to secrete large amounts of EPS, and was identified as <em>Pseudoalteromonas elyakovii</em> based on 16S rDNA analysis. The EPS, P-ArcPo 15, was purified by protease treatment and gel filtration chromatography. The purified EPS (P-ArcPo 15) had a molecular mass of 1.7 × 107 Da, and its infrared spectrum showed absorption bands of hydroxyl and carboxyl groups. The principal sugar components of P-ArcPo 15 were determined to be mannose and galacturonic acid, in the ratio of 3.3:1.0. The cryoprotective properties of P-ArcPo 15 were characterized by an <em>Escherichia coli</em> viability test. In the presence of 0.5%(w/v) EPS, the survival percentage of <em>E. coli</em> cells was as high as 94.19 ± 7.81% over five repeated freeze-thaw cycles. These biochemical characteristics suggest that the EPS P-ArcPo 15 may be useful in the development of cryoprotectants for biotechnological purposes, and we therefore assessed the utility of this novel cryoprotective EPS.-
dc.languageEnglish-
dc.subjectBiochemistry & Molecular Biology-
dc.subjectBiotechnology & Applied Microbiology-
dc.subject.classificationAraon-
dc.titleCryoprotective properties and preliminary characterization of exopolysaccharide (P-Arcpo 15) produced by the Arctic bacterium Pseudoalteromonas elyakovii Arcpo 15-
dc.typeArticle-
dc.identifier.bibliographicCitationKim, Sung Jin, et al. 2016. "Cryoprotective properties and preliminary characterization of exopolysaccharide (P-Arcpo 15) produced by the Arctic bacterium Pseudoalteromonas elyakovii Arcpo 15". <em>Preparative Biochemistry and Biotechnology</em>, 46(3): 261-266.-
dc.citation.titlePreparative Biochemistry and Biotechnology-
dc.citation.volume46-
dc.citation.number3-
dc.citation.page261-266.-
dc.identifier.doi10.1080/10826068.2015.1015568-
dc.coverage.x73.3°N-
dc.coverage.x82.3°N-
dc.coverage.y173°E-
dc.coverage.y153°W-
dc.subject.keywordArctic bacterium-
dc.subject.keywordCarbohydrate CPAs-
dc.subject.keywordCryoprotectant-
dc.subject.keywordCryoprotective properties-
dc.subject.keywordExopolysaccharide-
dc.subject.keywordPseudoalteromonas elyakovii-
dc.subject.keywordAraon-
dc.coverage.degreeX73.3-
dc.coverage.degreeX82.3-
dc.coverage.degreeY173-
dc.coverage.degreeY-153-
dc.identifier.scopusid2-s2.0-84968626566-
dc.identifier.wosid000374887000007-
Appears in Collections  
2011-2016, Exploration of Future Resources in The Polar Oceans and Study on Their Utilization (K-POD) (11-16) / Yim; Joung Han (PM11090; PM12030; PM13030; PM14050; PM15050)
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