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Optimization of Physical Factor for amylase Production by Arthrobacter sp. by Response Surface Methodology

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Title
Optimization of Physical Factor for amylase Production by Arthrobacter sp. by Response Surface Methodology
Authors
Kim, Hyun-do
Im, Young-kum
Choi, Jong-il
Han, Se Jong
Subject
Engineering
Keywords
Antarctic microorganism; Amylase; Response Surface Methodology
Issue Date
2016
Citation
Kim, Hyun-do, et al. 2016. "Optimization of Physical Factor for amylase Production by Arthrobacter sp. by Response Surface Methodology". Korean Chem. Eng. Res., 54(1): 140-144.
Abstract
In this study, the physical factors for amylase production by Arthrobacter sp. were optimized using response surface methodology(RSM). Antarctic microorganism Arthrobacter sp. PAMC 27388 was obtained from the Polar and Alpine Microbial Collection(PAMC) at the Korea Polar Research Institute. This microorganism was confirmed for the excretion of amylase with Lugol`s solution. The amylase activity was after flask culture was as low as 1.66 mU/L before optimization. The physical factors including the inoculum volume, the initial culture pH, and the medium volume were chosen to be optimized for the enhanced amylase production. The calculated results using RSM indicate that the optimal physical factors were 2.49 mL inoculum volume, 6.85 pH and 42.87 mL medium volume with a predicted amylase production of 2.84 mU/L. The experimentally obtained amylase activity was 2.50 mU/L, which was a 150% increase compared to the level before optimization.
DOI
http://dx.doi.org/10.9713/kcer.2016.54.1.140
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