Optimization of Medium for Protease Production by Enterobacteriaceae sp. PAMC 25617 by Response Surface Methodology

Abstract

This study was conducted to optimize the medium composition for cold-adaptive protease production of <em>Enterobacteriaceae</em> sp. by response surface methodology (RSM). Yeast extract, and TritonX-100 were identified as the significant factors affecting protease from one-factor-at-a-time method. RSM studies for optimizing protease production of <em>Enterobacteriaceae </em>sp. have been carried out for three parameters including yeast extract concentration, TritonX-100 concentration, and culture pH. These significant factors were optimized as 6.690 g/L yeast extract, 0.018 g/L Triton™ X-10, and pH 6.677. The experimentally obtained protease activity was 8.03 U /L, and it became 1.5-fold increase before optimization.