Investigation of the Susceptibility of Arctic Arthrobacter sp.PAMC 25486 to Mutagens

Abstract

This study was conducted to investigate the sensitivity of Arthrobacter sp. PAMC 25486 to various mutagens. γ-ray, UV-ray, Ethyl methane sulfonate (EMS) and hydrogen peroxide (H2O2) were used as mutagen, and the survival rate of Arthrobacter sp. was measured at various doses of γ-ray and UV-ray, and concentrations of EMS and H2O2. Decimal reduction dose (D10 value) of Arthrobacter sp. was determined 370 Gy for a gamma irradiation treatment, 0.019 J for a UV ray, 2.5 mM for EMS, and 230 mM for H2O2. This result will be applied for the development of superior mutant strain of Arctic bacteria producing valuable compounds.