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Controlled dehydration improves the diffraction quality of two RNA crystals

Cited 2 time in wos
Cited 2 time in scopus

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dc.contributor.authorPark, HaJeung-
dc.contributor.authorTran, Tuan-
dc.contributor.authorLee, Jun Hyuck-
dc.contributor.authorPark, Hyun-
dc.contributor.authorDisney, Matthew D.-
dc.date.accessioned2017-08-03T13:40:01Z-
dc.date.available2017-08-03T13:40:01Z-
dc.date.issued2016-
dc.description.abstractBackground: Post-crystallization dehydration methods, applying either vapor diffusion or humidity control devices, have been widely used to improve the diffraction quality of protein crystals. Despite the fact that RNA crystals tend to diffract poorly, there is a dearth of reports on the application of dehydration methods to improve the diffraction quality of RNA crystals. Results: We use dehydration techniques with a Free Mounting System (FMS, a humidity control device) to recover the poor diffraction quality of RNA crystals. These approaches were applied to RNA constructs that model various RNA-mediated repeat expansion disorders. Conclusion: The method we describe herein could serve as a general tool to improve diffraction quality of RNA crystals to facilitate structure determinations.-
dc.languageEnglish-
dc.subjectBiophysics-
dc.titleControlled dehydration improves the diffraction quality of two RNA crystals-
dc.typeArticle-
dc.identifier.bibliographicCitationPark, HaJeung, et al. 2016. "Controlled dehydration improves the diffraction quality of two RNA crystals". <em>BMC Structural Biology</em>, 16(1): 19.-
dc.citation.titleBMC Structural Biology-
dc.citation.volume16-
dc.citation.number1-
dc.citation.page19-
dc.identifier.doi10.1186/s12900-016-0069-1-
dc.subject.keywordPost-crystallization modification-
dc.subject.keywordRNA-
dc.subject.keywordCrystal dehydration-
dc.subject.keywordFree mounting system-
dc.subject.keywordRepeat expansion-
dc.identifier.scopusid2-s2.0-84993989968-
dc.identifier.wosid000386961200001-
Appears in Collections  
2014-2016, Antarctic Organisms: Cold-Adaptation Mechanism and Its Application (14-16) / Park; Hyun (PE14070; PE15070; PE16070)
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