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Characterization of β-N-acetylglucosaminidase from a marine Pseudoalteromonas sp. for application in N-acetyl-glucosamine production

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Title
Characterization of β-N-acetylglucosaminidase from a marine Pseudoalteromonas sp. for application in N-acetyl-glucosamine production
Authors
Park, Ha Ju
Yim, Joung Han
Park, Hyun
Kim, Dockyu
Subject
Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
Keywords
β-N-Acetylglucosaminidase; Arctic marine; Cold-active; Exochitinase; N-acetyl-glucosamine; Psychrotolerant
Issue Date
2016
Citation
Park, Ha Ju., et al. 2016. Characterization of β-N-acetylglucosaminidase from a marine Pseudoalteromonas sp. for application in N-acetyl-glucosamine production. Perparative Biochemistry and Biothenology, 46(8): 764?771.
Abstract
The psychrotolerant Pseudoalteromonas issachenkonii PAMC 22718 was isolated for its high exo-acting chitinase activity in the Kara Sea, Arctic. An exo-acting chitinase (W-Chi22718) was homogeneously purified from the culture supernatant of PAMC 22718 and the molecular weight of W-Chi22718 was estimated to be ~112 kDa. Because of its β-N-acetylglucosaminidase activity, W-Chi22718 was able to produce N-acetyl-D-glucosamine monomers from chitin oligosaccharide substrates. W-Chi22718 displayed chitinase activity from 0?37 °C (optimal temperature of 30 °C) and maintained activity between pH 6.0 and 9.0 (optimal pH of 7.6). W-Chi22718 exhibited a relative activity of 13 and 35% of the maximal activity at 0 and 10 °C, respectively, which is comparable to the activities of previously characterized, cold-adapted, bacterial chitinases. W-Chi22718 activity was enhanced by K+, Ca2+, and Fe2+, but inhibited by Cu2+and sodium dodecyl sulfate. We found that W-Chi22718 can produce large amounts of N-acetyl-D-glucosamines from colloidal chitin when combined with the previously characterized cold-active endochitinase W-Chi21702. Genome sequencing revealed that the corresponding gene (chi22718_IV) is 2,856 bp and encodes a 951 amino acid protein with a calculated molecular weight of ~102 kDa.
DOI
10.1080/10826068.2015.1135459
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