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Cold adaptation of a psychrophilic chaperoninfrom Psychrobacter sp. and its application for heterologousprotein expression

Cited 4 time in scopus
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Title
Cold adaptation of a psychrophilic chaperoninfrom Psychrobacter sp. and its application for heterologousprotein expression
Authors
Kim, Han-Woo
Wi, Ah Ram
Jeon, Byoung Wook
Lee, Jun Hyuck
Shin, Seung Chul
Park, Hyun
Jeon, Sung-Jong
Subject
Biotechnology & Applied Microbiology
Keywords
Chaperonin; Cold adaptation; Esterase; GroEL; Psychrobacter sp.; Psychrophilic bacterium
Issue Date
2015
Citation
Kim, Han-Woo, et al. 2015. "Cold adaptation of a psychrophilic chaperoninfrom Psychrobacter sp. and its application for heterologousprotein expression". Biotechnology Letters,, 37: 1887-1893.
Abstract
Objectives A chaperonin, PsyGroELS, from the Antarctic psychrophilic bacterium Psychrobacter sp. PAMC21119, was examined for its role in cold adaptation when expressed in a mesophilic Escherichia coli strain. Results Growth of E. coli harboring PsyGroELS at 10 C was increased compared to the control strain. A co-expression system using PsyGroELS was developed to increase productivity of the psychrophilic enzyme PsyEst9. PsyEst9 was cloned and expressed using three E. coli variants that co-expressed GroELS from PAMC21119, E. coli, or Oleispira antarctica RB8T. Co-expression with PsyGroELS was more effective for the production of PsyEst9 compared tothe other chaperonins. Conclusion PsyGroELS confers cold tolerance to E. coli, and shows potential as an effective coexpression system for the stable production of psychrophilic proteins.
DOI
http://dx.doi.org/10.1007/s10529-015-1860-y
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