Cold adaptation of a psychrophilic chaperoninfrom Psychrobacter sp. and its application for heterologousprotein expression
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- Cold adaptation of a psychrophilic chaperoninfrom Psychrobacter sp. and its application for heterologousprotein expression
- Kim, Han-Woo
Wi, Ah Ram
Jeon, Byoung Wook
Lee, Jun Hyuck
Shin, Seung Chul
- Biotechnology & Applied Microbiology
- Chaperonin; Cold adaptation; Esterase; GroEL; Psychrobacter sp.; Psychrophilic bacterium
- Issue Date
- Kim, Han-Woo, et al. 2015. "Cold adaptation of a psychrophilic chaperoninfrom Psychrobacter sp. and its application for heterologousprotein expression". Biotechnology Letters,, 37: 1887-1893.
- Objectives A chaperonin, PsyGroELS, from the Antarctic psychrophilic bacterium Psychrobacter sp.
PAMC21119, was examined for its role in cold adaptation when expressed in a mesophilic Escherichia coli strain. Results Growth of E. coli harboring PsyGroELS at
10 C was increased compared to the control strain. A co-expression system using PsyGroELS was developed to increase productivity of the psychrophilic enzyme PsyEst9. PsyEst9 was cloned and expressed
using three E. coli variants that co-expressed GroELS from PAMC21119, E. coli, or Oleispira antarctica RB8T. Co-expression with PsyGroELS was more effective for the production of PsyEst9 compared tothe
other chaperonins. Conclusion PsyGroELS confers cold tolerance to E. coli, and shows potential as an effective coexpression system for the stable production of psychrophilic proteins.
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