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Constitutive expression of DaCBF7, an Antarctic vascular plant Deschampsia antarctica CBF homolog, resulted in improved cold tolerance in transgenic rice plants

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Title
Constitutive expression of DaCBF7, an Antarctic vascular plant Deschampsia antarctica CBF homolog, resulted in improved cold tolerance in transgenic rice plants
Authors
Byun, Mi Young
Lee, Jungeun
Cui, Li Hua
Kang, Yoonjee
Oh, Tae Kyung
Park, Hyun
Lee, Hyoungseok
Kim, Woo Taek
Subject
Biochemistry & Molecular Biology; Plant Sciences
Keywords
C-repeat/DRE binding factor; Cold stress tolerance; Deschampsia antarctica; RNA-seq analysis; Transgenic rice (Oryza sativa) plant; Antarctic
Issue Date
2015
Citation
Byun, Mi Young., et al. 2015. Constitutive expression of DaCBF7, an Antarctic vascular plant Deschampsia antarctica CBF homolog, resulted in improved cold tolerance in transgenic rice plants. Plant Science, 236: 61?74.
Abstract
Deschampsia antarctica is an Antarctic hairgrass that grows on the west coast of the Antarctic penin-sula. In this report, we have identified and characterized a transcription factor, D. antarctica C-repeatbinding factor 7 (DaCBF7), that is a member of the monocot group V CBF homologs. The protein con-tains a single AP2 domain, a putative nuclear localization signal, and the typical CBF signature. DaCBF7,like other monocot group V homologs, contains a distinct polypeptide stretch composed of 43 aminoacids in front of the AP2 motif. DaCBF7 was predominantly localized to nuclei and interacted withthe C-repeat/dehydration responsive element (CRT/DRE) core sequence (ACCGAC) in vitro. DaCBF7 wasinduced by abiotic stresses, including drought, cold, and salinity. To investigate its possible cellular rolein cold tolerance, a transgenic rice system was employed. DaCBF7-overexpressing transgenic rice plants(Ubi:DaCBF7) exhibited markedly increased tolerance to cold stress compared to wild-type plants withoutgrowth defects; however, overexpression of DaCBF7 exerted little effect on tolerance to drought or saltstress. Transcriptome analysis of a Ubi:DaCBF7 transgenic line revealed 13 genes that were up-regulatedin DaCBF7-overexpressing plants compared to wild-type plants in the absence of cold stress and in short-or long-term cold stress. Five of these genes, dehydrin, remorin, Os03g63870, Os11g34790, and Os10g22630,contained putative CRT/DRE or low-temperature responsive elements in their promoter regions. Theseresults suggest that overexpression of DaCBF7 directly and indirectly induces diverse genes in transgenicrice plants and confers enhanced tolerance to cold stress.
DOI
10.1016/j.plantsci.2015.03.020
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