KOPRI Repository

Controlled dehydration improves the diffraction quality of two RNA crystals

Cited 0 time in scopus
Metadata Downloads
Title
Controlled dehydration improves the diffraction quality of two RNA crystals
Authors
Park, HaJeung
Tran, Tuan
Lee, Jun Hyuck
Park, Hyun
Disney, Matthew D.
Subject
Biophysics
Keywords
Post-crystallization modification; RNA; Crystal dehydration; Free mounting system; Repeat expansion
Issue Date
2016
Citation
Park, HaJeung, et al. 2016. "Controlled dehydration improves the diffraction quality of two RNA crystals". BMC Structural Biology, 16(1): 19.
Abstract
Background: Post-crystallization dehydration methods, applying either vapor diffusion or humidity control devices, have been widely used to improve the diffraction quality of protein crystals. Despite the fact that RNA crystals tend to diffract poorly, there is a dearth of reports on the application of dehydration methods to improve the diffraction quality of RNA crystals. Results: We use dehydration techniques with a Free Mounting System (FMS, a humidity control device) to recover the poor diffraction quality of RNA crystals. These approaches were applied to RNA constructs that model various RNA-mediated repeat expansion disorders. Conclusion: The method we describe herein could serve as a general tool to improve diffraction quality of RNA crystals to facilitate structure determinations.
DOI
http://dx.doi.org/10.1186/S12900-16-69-1
Files in This Item
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse