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Temperature-dependenet transcriptome analysis of the arctic Chlamydomonas sp.

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Title
Temperature-dependenet transcriptome analysis of the arctic Chlamydomonas sp.
Authors
Lee, Jungeun
Kim, Sanghee
Cho, Sung-Mi
Park, Hyun
Keywords
Arctic; Cold; Chlamydomonas; Transcriptome
Issue Date
2016
Citation
Lee, Jungeun, et al. 2016. Temperature-dependenet transcriptome analysis of the arctic Chlamydomonas sp.. 11th International Congress on Extremophiles. Japan. 2016.09.12.-16.
Abstract
Chlamydomonas sp. is widely present in the Earth’s aquatic ecosystem and is worth as a model species based on environmental factors related to environmental stresses. As a part of a study on the environmental adaptation mechanisms that exist in polar Chlamydomonas, we selected an Arctic Chlamydomonas sp. strain from the KOPRI culture collection of polar microorganisms (KCCPM, KNF0024), which had been isolated from ice-covered freshwater from the Arctic. This strain shows psychrophilic growth. To explore its adaptation strategy in freezing environments and identify the genes related cold acclimation, we investigated its temperature-dependent transcriptome changes. Uni-gene sets of this strain were established through de-novo assembly. The assembled contigs were annotated and functionally classified according to the biological functions. We examined the candidate target genes whose expression differs in accordance with the temperature, clustered them by expression patterns. In addition, we compared the temperature-dependent transcriptome response between this Artic Chlamydomonas sp. with those of Chlamydomonas reinhardtii, a mesophilic species. This dataset would be a valuable resource to understand the molecular mechanism behind Chlamydomonas’ adaptation under extreme and changing environment.
Conference Name
11th International Congress on Extremophiles
Conference Place
Japan
Conference Date
2016.09.12.-16
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