KOPRI Repository

Starch based polyhydroxybutyrate production in engineered Escherichia coli

Cited 11 time in scopus
Metadata Downloads
Title
Starch based polyhydroxybutyrate production in engineered Escherichia coli
Authors
Bhatia, Shashi Kant
Shim, Young-Ha
Jeon, Jong-Min
Brigham, Christopher J.
Kim, Yong-Hyun
Kim, Hyun Joong
Seo, Hyung Min
Lee, Ju Hee
Kim, Jung-Ho
Yi, Da-Hye
Lee, Yoo Kyung
Yang, Yung-Hun
Subject
Biotechnology & Applied Microbiology; Engineering
Keywords
Amylase; Biodegradable; Panibacillus sp.; Polyhydroxybutyrate; Ralstonia eutropha
Issue Date
2014
Citation
Bhatia, Shashi Kant, et al. 2014. "Starch based polyhydroxybutyrate production in engineered Escherichia coli". Bioprocess Biosyst Eng., 38: 1479-1484.
Abstract
Every year, the amount of chemosynthetic plastic accumulating in the environment is increasing, and significant time is required for decomposition. Bio-based, biodegradable plastic is a promising alternative, but its production is not yet a cost effective process. Decreasing the production cost of polyhydroxyalkanoate by utilizing renewable carbon sources for biosynthesis is an important aspect of commercializing this biodegradable polymer. An Escherichia coli strain that expresses a functional amylase and accumulate polyhydroxybutyrate (PHB), was constructed using different plasmids containing the amylase gene of Panibacillus sp. and PHB synthesis genes from Ralstonia eutropha. This engineered strain can utilize starch as the sole carbon source. The maximum PHB production (1.24 g/L) was obtained with 2 % (w/v) starch in M9 media containing 0.15 % (w/v) yeast extract and 10 mM glycine betaine. The engineered E. coli SKB99 strain can accumulate intracellular PHB up to 57.4 % of cell dry mass.
DOI
http://dx.doi.org/10.1007/s00449-015-1390-y
Files in This Item
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse