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Morphological characteristics and complete sequences of two cold-active Pseudoalteromonas phages

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dc.contributor.authorHwang, Chung Yeon-
dc.contributor.authorYoon, Soo Jung-
dc.contributor.authorNam, Seung-Il-
dc.contributor.authorLee, Hong Kum-
dc.coverage.spatialMendeleev Ridge-
dc.date.accessioned2017-08-03T18:00:32Z-
dc.date.available2017-08-03T18:00:32Z-
dc.date.issued2016-
dc.description.abstractCold-active bacteriophages are generally defined as bacterial viruses that are capable of infection and production at low temperature (≤ 4°C). Several studies of cold-active bacteriophages isolated from aquatic environments have been reported. However, characteristics of cold-active bacteriophage-host systems in deep-sea sediments are still poorly understood, despite high viral abundance maintain in persistently low temperature. Two bacteriophages, φCA1 and φCA2, were isolated from a deep-sea sediment core taken from the Mendeleev Ridge in the western Arctic Ocean. Host bacteria of φCA1 and φCA2 were affiliated with the genus <em>Pseudoalteromonas</em> based on 16S rRNA gene sequences. The <em>Pseudoalteromonas</em> bacteriophages of φCA1 and φCA2 turned out to exhibit successive propagations at low temperature of 1°C, indicating that they are cold-active ones. Each phage has a contractile tail, which is a morphological feature of the family <em>Myoviridae</em>. To find genomic characteristics of the cold-active phages, a complete genome sequence of each phage was obtained by Illumina MiSeq sequencing technology. The genome sizes of φCA1 and φCA2 were 36,825 bp and 36,989 bp, respectively, with an identical G+C content of 43.1 mol%. Details of genomic content of the cold-active phages and their relatedness with other phages will be provided in a poster presentation. This work was supported by a grant of Korea Polar Research Institute (PE16062).-
dc.languageEnglish-
dc.titleMorphological characteristics and complete sequences of two cold-active Pseudoalteromonas phages-
dc.typePoster-
dc.identifier.bibliographicCitationHwang, Chung Yeon, et al. 2016. Morphological characteristics and complete sequences of two cold-active Pseudoalteromonas phages. International Society for Microbial Ecology. Montreal. 2016.08.21.-26.-
dc.citation.conferenceDate2016.08.21.-26-
dc.citation.conferenceNameInternational Society for Microbial Ecology-
dc.citation.conferencePlaceMontreal-
dc.subject.keyword<em>Pseudoalteromonas</em>-
dc.subject.keywordCold-active virus-
dc.subject.keywordGenome sequence-
Appears in Collections  
2014-2016, Characteristics of gas hydrate and reconstruction of paleo-environment changes in the western Arctic (14-16) / Nam, Seung-Il (PE14062; PE15062; PE16062)
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