Role of crustacean hyperglycemic hormone (CHH) in the environmental stressor-exposed intertidal copepod Tigriopus japonicus
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- Role of crustacean hyperglycemic hormone (CHH) in the environmental stressor-exposed intertidal copepod Tigriopus japonicus
- Kim, Bo-Mi
- Biochemistry & Molecular Biology; Endocrinology & Metabolism; Toxicology; Zoology
- Copepod; Tigriopus japonicus; Crustacean hyperglycemic hormone; CHH; WAFs; Oil spill; Water-accommodated fraction
- Issue Date
- Kim, Bo-Mi., et al. 2013. Role of crustacean hyperglycemic hormone (CHH) in the environmental stressor-exposed intertidal copepod Tigriopus japonicus. Comparative Biochemistry and Physiology, Part C, 158: 131-141.
- To identify and characterize CHH (TJ-CHH) gene in the copepod Tigriopus japonicus, we analyzed the full-length
cDNA sequence, genomic structure, and promoter region. The full-length TJ-CHH cDNA was 716 bp in length,
encoding 136 amino acid residues. The deduced amino acid sequences of TJ-CHH showed a high similarity of
the CHH mature domain to other crustaceans. Six conserved cysteine residues and five conserved structural
motifs in the CHH mature peptide domain were also observed. The genomic structure of the TJ-CHH gene
contained three exons and two introns in its open reading frame (ORF), and several transcriptional elements
were detected in the promoter region of the TJ-CHH gene. To investigate transcriptional change of TJ-CHH
under environmental stress, T. japonicus were exposed to heat treatment, UV-B radiation, heavy metals, and
water-accommodated fractions (WAFs) of Iranian crude oil. Upon heat stress, TJ-CHH transcripts were elevated
at 30 °C and 35 °C for 96 h in a time-course experiment. UV-B radiation led to a decreased pattern of the TJ-CHH
transcript 48 h and more after radiation (12 kJ/m2). After exposure of a fixed dose (12 kJ/m2) in a time-course
experiment, TJ-CHH transcript was down-regulated in time-dependent manner with a lowest value at 12 h.
However, the TJ-CHH transcript level was increased in response to five heavy metal exposures for 96 h. Also,
the level of the TJ-CHH transcript was significantly up-regulated at 20% of WAFs after exposure to WAFs for
48 h and then remarkably reduced in a dose-dependent manner. These findings suggest that the enhanced
TJ-CHH transcript level is associated with a cellular stress response of the TJ-CHH gene as shown in decapod
crustaceans. This study is also helpful for a better understanding of the detrimental effects of environmental
changes on the CHH-triggered copepod metabolism.
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