Lobaric Acid Inhibits VCAM-1 Expression in TNF-α-Stimulated Vascular Smooth Muscle Cells via Modulation of NF-κB and MAPK Signaling Pathways
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- Lobaric Acid Inhibits VCAM-1 Expression in TNF-α-Stimulated Vascular Smooth Muscle Cells via Modulation of NF-κB and MAPK Signaling Pathways
- Kwon, Ii-Seul
Yim, Joung Han
Lee, Hong Kum
- Biochemistry & Molecular Biology; Pharmacology & Pharmacy
- Lobaric acid; Atherosclerosis; VCAM-1; MAPK; NF-κB; MOVAS-1
- Issue Date
- Kwon, Ii-Seul., et al. 2016. Lobaric Acid Inhibits VCAM-1 Expression in TNF-α-Stimulated Vascular Smooth Muscle Cells via Modulation of NF-κB and MAPK Signaling Pathways. Biomelecules and Therapeutics, 24(1): 25-32.
- Lichens have been known to possess multiple biological activities, including anti-proliferative and anti-inflammatory activities.
Vascular cell adhesion molecule-1 (VCAM-1) may play a role in the development of atherosclerosis. Hence, VCAM-1 is a possible
therapeutic target in the treatment of the inflammatory disease. However, the effect of lobaric acid on VCAM-1 has not yet been
investigated and characterized. For this study, we examined the effect of lobaric acid on the inhibition of VCAM-1 in tumor necrosis
factor-alpha (TNF-α)-stimulated mouse vascular smooth muscle cells. Western blot and ELISA showed that the increased expression
of VCAM-1 by TNF-α was significantly suppressed by the pre-treatment of lobaric acid (0.1-10 μg/ml) for 2 h. Lobaric acid
abrogated TNF-α-induced NF-κB activity through preventing the degradation of IκB and phosphorylation of extracellular signalregulated
kinases (ERK), c-Jun N-terminal kinases (JNK), and p38 mitogen activated protein (MAP) kinase. Lobaric acid also
inhibited the expression of TNF-α receptor 1 (TNF-R1). Overall, our results suggest that lobaric acid inhibited VCAM-1 expression
through the inhibition of p38, ERK, JNK and NF-κB signaling pathways, and downregulation of TNF-R1 expression. Therefore, it
is implicated that lobaric acid may suppress inflammation by altering the physiology of the atherosclerotic lesion.
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