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Production and characterization of medium-chain-length polyhydroxyalkanoates copolymer from Arctic psychrotrophic bacterium Pseudomonas sp. PAMC 28620

Cited 3 time in scopus
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Title
Production and characterization of medium-chain-length polyhydroxyalkanoates copolymer from Arctic psychrotrophic bacterium Pseudomonas sp. PAMC 28620
Other Titles
북극에서 분리된 내냉성 세균 슈도모나스 PAMC 28620에서 합성한 중간사슬길이의 polyhydroxyalkanoates copolymer의 생산과 특성분석
Authors
Ganesan Sathiyanarayanan
Yang, Yung-Hun
Kim, Yun-Gon
Lee, Yoo Kyung
Kim, Junyoung
Jeon, Jong-Min
Song, Hun-Suk
Shashi Kant Bhatia
Subject
Biochemistry & Molecular Biology; Chemistry
Keywords
Arctic Pseudomonas; Copolymer; Fermentation; Glycerol; Polyhydroxyalkanoates; Polymer Science
Issue Date
2017
Citation
Ganesan Sathiyanarayanan, et al. 2017. "Production and characterization of medium-chain-length polyhydroxyalkanoates copolymer from Arctic psychrotrophic bacterium Pseudomonas sp. PAMC 28620". INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 97(1): 710-720.
Abstract
Arctic psychrotrophic bacterium Pseudomonas sp. PAMC 28620 was found to produce a distinctive medium-chain-length polyhydroxyalkanoates (MCL-PHAs) copolymer when grown on structurally unrelated carbon sources including glycerol. The maximum MCL-PHAs copolymer yield was obtained about 52.18 ± 4.12% from 7.95 ± 0.66 g/L of biomass at 144 h of fermentation when 3% glycerol was used as sole carbon and energy source during the laboratory-scale bioreactor process. Characterization of the copolymer was carried out using fourier transform infrared spectroscopy (FTIR), gas chromatography-mass spectrometry (GC-MS), proton (1H) and carbon (13C) nuclear magnetic resonance spectroscopy (NMR), gel permeation chromatography (GPC), differential scanning calorimeter (DSC) and thermo-gravimetric analysis (TGA). The copolymer produced by Pseudomonas sp. PAMC 28620 consisting of four PHA monomers and identified as 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxydodecanoate (3HDD) and 3-hydroxytetradecanoate (3HTD). An average molecular weight of the copolymer was found approximately 30.244 kDa with polydispersity index (PDI) value of 2.05. Thermal analysis showed the produced MCL-PHAs copolymer to be low-crystalline (43.73%) polymer with great thermal stability, having the thermal decomposition temperature of 230 °C to 280 °C, endothermic melting temperature (Tm) of 172.84 °C, glass transition (Tg) temperature of 3.99 °C, and apparent melting enthalpy fusion (ΔHm) about 63.85 J.g-1.
URI
http://pamc.kopri.re.kr/strain/PAMC%2028620
DOI
http://dx.doi.org/10.1016/j.ijbiomac.2017.01.053
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