Production of Antifreeze Protein from Antarctic Bacterium Flavobacteriumfrigoris PS1 by using Fed-batch Culture of Recombinant Pichiapastoris
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- Production of Antifreeze Protein from Antarctic Bacterium Flavobacteriumfrigoris PS1 by using Fed-batch Culture of Recombinant Pichiapastoris
- Other Titles
- 재조합 Pichia pastoris의 유가식 배양을 통한 남극세균 Flavobacteriumfrigoris PS1 유래 결빙방지단백질의 생산
- Kim, Eun Jae
Han, Se Jong
Kim, Hak Jun
Lee, Sung Gu
Lee, Jun Hyuck
- Antarctica; Antifreeze protein; Fed-batch fermentation; Ice-binding protein
- Issue Date
- Kim, Eun Jae, et al. 2014. "Production of Antifreeze Protein from Antarctic Bacterium Flavobacteriumfrigoris PS1 by using Fed-batch Culture of Recombinant Pichiapastoris". KSBB Journal, 29(4): 303-306.
- Antifreeze proteins (AFP) inhibit ice growth to permit the survival of polar organisms in the cold environments. The recombinant AFP from an Antarctic bacterium, Flavobacterium frigoris PS1, FfIBP (Flavobacterium frigoris ice-binding protein), was produced using Pichia pastoris expression system. The optimum fermentation temperature (30°C) and pH (5) for FfIBP production was determined using a fed-batch culture system. The maximal cell density and purified FfIBP were 112 g/L and 70 mg/L, respectively. The thermal hysteresis (TH) activity (0.85℃) of FfIBP obtained using a glycerol-methanol fed-batch culture system was 2-fold higher than that of the LeIBP (Leucosporidium ice-binding protein). This work allows for large-scale production of FfIBP, which could be extended to further application studies using recombinant AFPs.
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