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Axenic culture of Gyrodinium impudicum strain KG03, a marine red-tide microalga that produces exopolysaccharide

Cited 10 time in scopus
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Title
Axenic culture of Gyrodinium impudicum strain KG03, a marine red-tide microalga that produces exopolysaccharide
Other Titles
세포외다당류를 생산하는 적조유발 미세조류 자이로디니움 임푸디쿰 KG03의 순수분리
Authors
Yim, Joung Han
Lee, Hong Kum
Subject
Microbiology
Keywords
Gyrodinium impudicum; axenic culture; cell growth; exopolysaccharide
Issue Date
2004
Citation
Yim, Joung Han, Lee, Hong Kum. 2004. "Axenic culture of Gyrodinium impudicum strain KG03, a marine red-tide microalga that produces exopolysaccharide". JOURNAL OF MICROBIOLOGY, 42(4): 305-314.
Abstract
An exopolysaccharide-producing microalgal dinoflagellate was isolated from a red-tide bloom and designated strain KG03. A bacteria-free culture of strain KG03 was achieved using a modified wash with phototaxis and antibiotic treatment. Combined treatment with neomycin and cephalosporin was the most effective for eliminating the bacteria associated with the microalgae. Strain KG03 was identified as Gyrodinium impudicum by analyzing the ITS regions of the 5.8S rDNA, 18S rDNA, morphological phenotype and fatty acid composition. The exopolysaccharide production and cell growth in a 300-ml photobioreactor were increased 2.7- and 2.4-fold, respectively, compared with that in a flask culture at the first isolation step.
URI
http://repository.kopri.re.kr/handle/201206/5938
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