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Diversity of Cold-Active Protease-Producing Bacteria from Arctic Terrestrial Diversity of Cold-Active Protease-Producing Bacteria from Arctic Terrestrial

Cited 12 time in scopus
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Title
Diversity of Cold-Active Protease-Producing Bacteria from Arctic Terrestrial Diversity of Cold-Active Protease-Producing Bacteria from Arctic Terrestrial
Other Titles
북극환경에서 농화배양에 의해 분리된 저온활성 단백질분해효소 생산균주의 다양성
Authors
Lee, Yung Mi
Lee, Hong Kum
Kim, Eun Hye
Cho, Kyeung Hee
Hong, Soon Gyu
Cho, Jang-Cheon
Yim, Joung Han
Subject
Microbiology
Keywords
Arctic; enrichment; protease
Issue Date
2010
Publisher
Springer
Citation
Lee, Yung Mi, et al. 2010. "Diversity of Cold-Active Protease-Producing Bacteria from Arctic Terrestrial Diversity of Cold-Active Protease-Producing Bacteria from Arctic Terrestrial". JOURNAL OF MICROBIOLOGY, 48(4): 426-432.
Abstract
A new approach for enrichment culture was applied to obtain cold-active protease-producing bacteria for marine and terrestrial samples from Svalbard, Norway. The method was developed for the enrichment of bacteria by long-term incubation at low temperatures in semi-solid agar medium containing meat pieces as the main source of carbon and energy. ZoBell and 0.1x nutrient broth were added for marine and terrestrial microorganisms, respectively, to supply basal elements for growth. One to three types of colonies were observed from each enrichment culture, indicating that specific bacterial species were enriched during the experimental conditions. Among 89 bacterial isolates, protease activity was observed from 48 isolates in the screening media containing skim milk. Good growth was observed at 4C and 10C while none of the isolates could grow at 37C. At low temperatures, enzyme activity was equal to or higher than activity at higher temperatures. Bacterial isolates were included in the genera Pseudoalteromonas (33 isolates), Arthrobacter (24 isolates), Pseudomonas (16 isolates), Psychrobacter (6 isolates), Sphingobacterium (6 isolates), Flavobacterium (2 isolates), Sporosarcina (1 isolate), and Stenotrophomonas (1 isolate). Protease activity was observed from Pseudoalteromonas (33 isolates), Pseudomonas (10 isolates), Arthrobacter (4 isolates), and Flavobacterium (1 isolate).
URI
http://repository.kopri.re.kr/handle/201206/6183
DOI
http://dx.doi.org/10.1007/s12275-010-0015-z
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