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Five hepatopancreatic and one epidermal chitinases from a pandalid shrimp (Pandalopsis japonica): Cloning and effects of eyestalk ablation on gene expression

Cited 21 time in scopus
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Title
Five hepatopancreatic and one epidermal chitinases from a pandalid shrimp (Pandalopsis japonica): Cloning and effects of eyestalk ablation on gene expression
Other Titles
갑각류에서의 hepatopancreatic와 epidermal chitinases 유전자 연구
Authors
Umme Salma
Park, Hyun
Kim, Hyun-Woo
Md. Hasan Uddowla
Baek, Hae-Ja
Kim, Bo Kwang
Kim, Jong Min
Kim, Meesun
Donald L. Mykles
Subject
Biochemistry & Molecular Biology; Zoology
Keywords
Chitinase; Decapoda; Exoskeleton; Molting; Pandalopsis japonica
Issue Date
2012
Publisher
Elsevier
Citation
Umme Salma, et al. 2012. "Five hepatopancreatic and one epidermal chitinases from a pandalid shrimp (Pandalopsis japonica): Cloning and effects of eyestalk ablation on gene expression". COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 161(3): 197-207.
Abstract
Six cDNAs encoding chitinase proteins in Pandalopsis japonica were isolated by using polymerase chain reaction(PCR) cloning methods and bioinformatic analysis of expressed sequence tags (ESTs). The cDNAs, designated Pj-Cht1, 2, 3A, 3B, 3C, and 4, encoded proteins ranging from 388 to 607 amino acid residues in length (43.61?67.62 kDa) and displayed a common structural organization: an N-terminal catalytic domain, a Thr/Pro-rich linker region, and either 0 (Pj-Cht2, 3A), 1 (Pj-Cht1, 3B, and 3C), or 2 (Pj-Cht4) C-terminal chitinbinding domain(s) (CBD). Pj-Cht1 and 2 lacked the 5′ end of the open reading frame (ORF);the other Pj-Chts contained the complete ORF. All known decapod crustacean chitinases were segregated into at least four groups based on phylogenetic analysis and domain organization. Group 1 chitinases, represented by Pj-Cht1, were most closely related to insect group I chitinases and may function in the digestion of the peritrophic membrane. Group 2 chitinases including Pj-Cht2 show different domain organizations and pI value from other chitinases and appear to function in degradation of the old exoskeleton during the premolt period. Group 3 chitinases, represented by Pj-Cht3A, 3B, and 3C, may function in digestion of chitin-containing food and defense against pathogens. Group 4 chitinases, represented by Pj-Cht4, have two CBDs and their functions are unknown. Five Pj-Chts (Pj-Cht1, 3A, 3B, 3C, and 4) are expressed in the hepatopancreas and intestine, whereas Pj-Cht2 is expressed in epidermis and SG/XO complex suggesting crustacean chitinases can be classified into two groups (hepatopancreatic and epidermal) based on the expression profile. Eyestalk ablation (ESA) down-regulated the hepatopancreatic chitinase expression (Pj-Cht1, 3A, and 3C);Pj-Cht3B expression was not significantly affected by ESA. By contrast, mRNA levels of Pj-Cht2 were significantly upregulated in 7 days post-ESA. Pj-Cht4 mRNA levels were too low for measu
URI
http://repository.kopri.re.kr/handle/201206/6227
DOI
http://dx.doi.org/10.1016/j.cbpb.2011.11.005
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