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Characteristics of cold-adaptive endochitinase from Antarctic bacterium Sanguibacter antarcticus KOPRI 21702

Cited 24 time in wos
Cited 28 time in scopus
Title
Characteristics of cold-adaptive endochitinase from Antarctic bacterium Sanguibacter antarcticus KOPRI 21702
Other Titles
남극 미생물 Sanguibacter antarcticus KOPRI 21702이 생산하는 저온적은 키틴분해효소의 특성
Authors
Park, Ha Ju
Lee, Hong Kum
Kim, In Hee
Kim, Dockyu
Kim, Ju Young
Kim, Il-Chan
Yim, Joung Han
Kim, Sung Jin
Lee, Chang-Eun
Subject
Biotechnology & Applied Microbiology
Keywords
AntarcticChitinolyticCold-activeEndochitinasePsychrotroph
Issue Date
2009
Publisher
ELSEVIER
Citation
Park, Ha Ju, et al. 2009. "Characteristics of cold-adaptive endochitinase from Antarctic bacterium Sanguibacter antarcticus KOPRI 21702". Enzyme and Microbial Technology, 45(1): 391-396.
Abstract
The psychrotrophic Sanguibacter antarcticus KOPRI 21702T, isolated from Antarctic seawater, produced a cold-adapted chitinolytic enzyme that is a new55 kDa family 18 chitinase (Chi21702). Chi21702 exhibited high activities toward pNP-(GlcNAc)2 and pNP-(GlcNAc)3 with no activity for pNP-GlcNAc, indicating that it prefers chitin chains longer than dimers, just as endochitinases do.Amixture of GlcNAc and GlcNAc2 was produced as a main product by Chi21702 activity from chitin oligosaccharides and swollen chitin, while less GlcNAc3 was produced. These results show that Chi21702 has an endochitinase activity, randomly hydrolyzing chitin at internal sites. Chi21702 displayed chitinase activity at 0?40 ?C (optimal temperature of 37 ?C), maintained its activity at pH 4?11 (optimal pH of 7.6). Interestingly, Chi21702 exhibited relative activities of 40% and 60% at 0 and 10 ?C, respectively, in comparison to 100% at 37 ?C, which is higher than those of the previously characterized, cold-adapted, chitinases from bacterial strains.
URI
https://repository.kopri.re.kr/handle/201206/6253
DOI
http://dx.doi.org/10.1016/j.enzmictec.2009.07.002
Type
Article
Indexed
SCI
Appears in Collections  
2006-2010, Procurement and utilization of polar genetic resources (06-10) / Lee, Hong Kum; Yim, Joung Han (PE06050, PE07050, PE08050, PE09050, PE10050)
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