KOPRI Repository

Analysis of ESTs and expression of two peroxiredoxins in the thermally stressed Antarctic bivalve Laternula elliptica

Cited 33 time in scopus
Metadata Downloads
Title
Analysis of ESTs and expression of two peroxiredoxins in the thermally stressed Antarctic bivalve Laternula elliptica
Other Titles
Antarctic bivalve Laternula elliptica의 발현유전체 분석
Authors
Park, Hyun
Ahn, In-Young
Kim, Meesun
Cheon, Jina
Kim, Hak Jun
Subject
Immunology; Marine & Freshwater Biology; Veterinary Sciences
Keywords
Antarctic; ESTs; Laternula; Peroxiredoxin; Thermal stress; Fisheries
Issue Date
2008
Publisher
Elsevier
Citation
Park, Hyun, et al. 2008. "Analysis of ESTs and expression of two peroxiredoxins in the thermally stressed Antarctic bivalve Laternula elliptica". Fish & Shellfish Immunology, 25(5): 550-559.
Abstract
A total of 2592 expressed sequenced tags (ESTs) were generated from a cDNA library of the Antarctic bivalve Laternula elliptica, which is an important species in Antarctic coastal ecosystems. A cluster of 1789 unique sequences was revealed, which consisted of 273 contigs and 1076 singletons. Based on BLAST searches, we identified 508 genes, 56 of which were functionally related to immunity and stress responses, including the peroxiredoxins (Prxs). Prxs comprise an antioxidant protein family with conserved catalytic redox-active cysteine residues. We characterised two full-length cDNAs of the genes that encode peroxiredoxin V (lePrxV) and peroxiredoxin VI (lePrxVI) in L. elliptica. The lePrxV cDNA contains a 480-bp open reading frame (ORF), which encodes 159 amino acids, including two conserved cysteine residues that are characteristic of the atypical 2-Cys subgroup of the Prx family. LePrxVI contains a 702-bp ORF, which encodes 233 amino acids, including one conserved cysteine residue that is characteristic of the 1-Cys subgroup of the Prx family. RT-PCR analysis revealed the presence of lePrxV and lePrxVI transcripts in all the examined tissues. In addition, real-time PCR analysis indicated significant increases in the levels of lePrxV and lePrxVI transcripts were induced by thermal stress. These results suggest that lePrxV and lePrxVI play protective roles against oxidative stress caused by thermal exposure.
URI
http://repository.kopri.re.kr/handle/201206/6259
DOI
http://dx.doi.org/10.1016/j.fsi.2008.07.017
Files in This Item
There are no files associated with this item.
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse