KOPRI Repository

Biphenyl hydroxylation enhanced by an engineered o xylene dioxygenase from Rhodococcus sp. strain DK17

Cited 6 time in scopus
Metadata Downloads
Title
Biphenyl hydroxylation enhanced by an engineered o xylene dioxygenase from Rhodococcus sp. strain DK17
Other Titles
Rhodococcus sp. strain DK17 유래 개량형 o-xylene dioxygenase에 의한 biphenyl hydroxylation 효과 상승
Authors
Kang, Beom Sik
Yoo, Miyoun
Zylstra, Gerben J.
Kim, Dockyu
Kim, Eungbin
Subject
Microbiology
Keywords
Rhodococcus; Rieske dioxygenase; aromatic hydroxylation; hydrophobic interaction
Issue Date
2011
Publisher
Elsevier
Citation
Kang, Beom Sik, et al. 2011. "Biphenyl hydroxylation enhanced by an engineered o xylene dioxygenase from Rhodococcus sp. strain DK17". Research in Microbiology, 162: 724-728.
Abstract
Hydroxylation of the non-growth substrate biphenyl by recombinant o-xylene dioxygenases from Rhodococcus sp. strain DK17 was studied through bioconversion experiments. The metabolites from the biphenyl hydroxylation by each enzyme were identified and quantified by gas chromatography-mass spectrometry. The L266F mutant enzyme produced much more 2 hydroxybiphenyl (2.43 vs. 0.1 μg/L) and 3 hydroxybiphenyl (1.97 vs. 0.03 μg/L) than the wild-type. Site-directed mutagenesis combined with structural and functional analyses indicated that hydrophobic interactions and shielding effects against water are important factors in the hydroxylation of biphenyl by the o xylene dioxygenase. The residue at position 266 plays a key role in coordinating the reaction.
URI
http://repository.kopri.re.kr/handle/201206/6303
DOI
http://dx.doi.org/10.1016/j.resmic.2011.04.013
Files in This Item
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse