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Isolation and spectral characterization of cadmium binding metallothionein

Cited 2 time in scopus
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Title
Isolation and spectral characterization of cadmium binding metallothionein
Other Titles
남극큰띠조개의 중금속 결합 단백질 분리 및 특성 연구
Authors
Park, Hyun
Ji, Jung Youn
Ahn, In-Young
Choi, Heeseon J.
Subject
Environmental Sciences & Ecology; Physical Geography
Keywords
Antarctic; Cd-thiolate complex; Laternula; MTs; bivalves
Issue Date
2008
Citation
Park, Hyun, et al. 2008. "Isolation and spectral characterization of cadmium binding metallothionein". ANTARCTIC SCIENCE, 20(1): 33-37.
Abstract
A cadmium (Cd)-binding protein was isolated and characterized from the Antarctic clam Laternula elliptica after experimental exposure to a high concentration of Cd. Cd-binding metallothioneins (MTs) in the cytosol were purified using a procedure based on gel permeation and ion-exchange chromatography. The purified MTs were recognized by MT antibodies in a Western blotting assay. MALDI-TOF MS analyses showed that the molecular mass of the purified MTs was 7.27 kDa, which is typical of MTs found in marine invertebrates. The Cd binding to MT, reflected by the redistribution of Cd ions, was monitored by spectrophotometry. The absorption spectra profiles indicated the presence of Cd-MT complexes, and a 4 nm red shift of the unresolved lowest energy-absorption band occurred when five equivalents of Cd (II) were incorporated.
URI
http://repository.kopri.re.kr/handle/201206/6325
DOI
http://dx.doi.org/10.1017/S0954102007000806
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