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Isolation and characterization of microsatellite loci in the Korean crayfish, Cambaroides similis and application to natural population analysis

Cited 4 time in scopus
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Title
Isolation and characterization of microsatellite loci in the Korean crayfish, Cambaroides similis and application to natural population analysis
Other Titles
한국가재 Cambaroides similis에서 microsatellite를 개발, 이를 이용한 natural polulation 분석
Authors
Dong-Ha
Jongwoo
Jae-Ho
Gi-Sik
Kim, Sanghee
Mi-Jin
Mi-Hyun
Subject
Cell Biology; Zoology
Keywords
Cambaroides similis; Korea; freshwater crayfish; microsatellite loci; population genetics
Issue Date
2011
Publisher
Taylor
Citation
Dong-Ha, et al. 2011. "Isolation and characterization of microsatellite loci in the Korean crayfish, Cambaroides similis and application to natural population analysis". animal cells and systems, 15(1): 37-43.
Abstract
The Korean freshwater crayfish, Cambaroides similis, has recently suffered from range reduction and habitatdegradation caused by environmental changes and water pollution. For the conservation and restoration of this species, it is necessary to understand the current population structures of Korean C. similis using estimation of their genetic variation. In this study, eight microsatellite loci were developed and characterized from 49 individuals collected from four locations: one population from Mt. Bukhan (BH) and three populations from Mt. Gwanak (GA) in Seoul, Korea. As a result, the number of alleles per locus ranged from 2 to 12. The observed heterozygosities and expected heterozygosities ranged from 0.000 to 0.833 and from 0.125 to 0.943, respectively, and the former values were significantly lower than the latter ones expected under the Hardy- Weinberg equilibrium. No significant linkage disequilibrium was revealed between any of the locus pairs after Bonferroni correction. From the pairwise Fst results over all samples, higher differentiation between GA-BH population pairs (mean 0.1789) was bserved than between GA population pairs (mean 0.0454). This was also supported by Mantel’s test showing that the genetic distances of these crayfish populations were significantly correlated with geographic distances.ssary to understand the current population structures of Korean C. similis using estimation of their genetic variation. In this study, eight microsatellite loci were developed and characterized from 49 individuals collected from four locations: one population from Mt. Bukhan (BH) and three populations from Mt. Gwanak (GA) in Seoul, Korea. As a result, the number of alleles per locus ranged from 2 to 12. The observed heterozygosities and expected heterozygosities ranged from 0.000 to 0.833 and from 0.125 to 0.943, respectively, and the former values were significantly lower than the latter ones expected under the Ha
URI
http://repository.kopri.re.kr/handle/201206/6370
DOI
http://dx.doi.org/10.1080/19768354.2011.555137
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