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Preliminary X-ray crystallographic analysis of the breakage-reunion domain of the GyrA subunit of DNA gyrase from Colwellia psychrerythraea strain 34H

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Title
Preliminary X-ray crystallographic analysis of the breakage-reunion domain of the GyrA subunit of DNA gyrase from Colwellia psychrerythraea strain 34H
Other Titles
Colwellia psychrerythraes strain 34 H의 GyrA 단백질의 X-선 분광학적 분석
Authors
Kim, Kyung Ha
Heo, Yong-Seok
Kim, Hyun-Kyung
Jung, Ha Yun
Hyoung, Ji Hye
Kim, Hak Jun
Kim, Yangmee
Lee, Ki Jeung
Han, Mi Ra
Subject
Biochemistry & Molecular Biology; Biophysics; Crystallography
Keywords
Colwellia psychrerythraea 34H; DNA gyrase; topoisomerase
Issue Date
2010
Publisher
crystallization communications
Citation
Kim, Kyung Ha, et al. 2010. "Preliminary X-ray crystallographic analysis of the breakage-reunion domain of the GyrA subunit of DNA gyrase from Colwellia psychrerythraea strain 34H". ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 66(4): 439-441.
Abstract
DNA gyrase is a type II topoisomerase that is essential for chromosome segregation and cell division owing to its ability to modify the topological forms of bacterial DNA. In this study, the N-terminal breakage?reunion domain of the GyrA subunit of DNA gyrase from Colwellia psychrerythraea 34H was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.60 A ° resolution using a synchrotron-radiation source. The crystal belonged to space group P212121, with unit-cell parameters a = 98.98, b = 101.56, c = 141.83 A. The asymmetric unit contained two molecules, with a corresponding VM of 3.18 A 3 Da and a solvent content of 59.9%.
URI
http://repository.kopri.re.kr/handle/201206/6392
DOI
http://dx.doi.org/10.1107/S1744309110005567
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