KOPRI Repository

Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of the N-terminal domain of DEAD-box RNA helicase from Staphylococcus su aureus strain Mu50

Cited 1 time in scopus
Metadata Downloads
Title
Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of the N-terminal domain of DEAD-box RNA helicase from Staphylococcus su aureus strain Mu50
Other Titles
Staphylococcus aureus Mu50의 DEAE-box RNA helicase의 클로닝, 정제 , 결정화 및 X-선 결정 예비 분석
Authors
Jung, Ha Yun
유기영
Kim, Yangmee
Lee, Soo Young
Heo, Yong-Seok
신환철
Kim, Hak Jun
백장미
임동원
김태오
Subject
Biochemistry & Molecular Biology; Biophysics; Crystallography
Keywords
ATP-dependent RNA-unwinding; DEAD-box RNA helicase; Staphylococcus aureus
Issue Date
2010
Publisher
International union of crystallography
Citation
Jung, Ha Yun, et al. 2010. "Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of the N-terminal domain of DEAD-box RNA helicase from Staphylococcus su aureus strain Mu50". ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 66(12): 1674-1676.
Abstract
DEAD-box helicases are enzymes with an ATP-dependent RNA-unwinding function that are involved in a variety of cellular processes including RNA splicing, ribosome biogenesis and RNA degradation. In this study, the N-terminal domain of DEAD-box RNA helicase from Staphylococcus aureus strain Mu50 was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.60??resolution using a synchrotron-radiation source. The crystal belonged to space group P1, with unit-cell parameters a = 70.81, b = 80.23, c = 86.25??, α = 69.54, β = 66.54, γ = 87.32°. The unit cell contained six molecules, with a corresponding V(M) of 2.91??(3)?Da(-1) and a solvent content of 56.1%.
URI
http://repository.kopri.re.kr/handle/201206/6393
DOI
http://dx.doi.org/10.1107/S1744309110043149
Files in This Item
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse