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A new method for microbial cultivation and its application to bacterial community analysis in Buus Nuur, Mongolia

Cited 2 time in scopus
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Title
A new method for microbial cultivation and its application to bacterial community analysis in Buus Nuur, Mongolia
Other Titles
새로운 미생물 배양법과 몽골 Buus Nuur에서 미생물 community 분석을 위한 적용
Authors
Jeong, Daun
Ahn, Tae Seok
Lee, Hong Kum
Yim, Joung Han
Yochan Joung
Slava S. Epstein
Seo, Eun-Young
Jeong, Daun
Subject
Marine & Freshwater Biology
Keywords
DGGE; bacterial diversity; improved culturability; soda Jake
Issue Date
2013
Publisher
Schweizerbart
Citation
Jeong, Daun, et al. 2013. "A new method for microbial cultivation and its application to bacterial community analysis in Buus Nuur, Mongolia". FUNDAMENTAL AND APPLIED LIMNOLOGY, 182: 171-181.
Abstract
We performed a comparative analysis ofbacterial di 、ers ity in a saline soda lake lIsing a novel clIltivation method termed the f? lter plate microbial σap method (FPMT), and conventional techniqlles together with DGGE (denaturing gradient gel eleclrophoresis). The new method allowed for cllltivat10n of representatives of se、en bacterial taxonomic grollps, incJuding seven noveJ species. This compares favorably to the reslIJts from cllltivation of representatives of four taxonomic groups and onJy one novel species using standard approaches. Neither culture collection matched the community composition revealed by DGGE but the FPMT method produced a more representative collection of strains. We conclude that FPMT is a powerω 1 device for improving bacterial c비turability and narrowing the gap between microbial diversity in nature and cllltivable microorganisms.ng gradient gel eleclrophoresis). The new method allowed for cllltivat10n of representatives of se、en bacterial taxonomic grollps, incJuding seven noveJ species. This compares favorably to the reslIJts from cllltivation of representatives of four taxonomic groups and onJy one novel species using standard approaches. Neither culture collection matched the community composition revealed by DGGE but the FPMT method produced a more representative collection of strains. We conclude that FPMT is a powerω 1 device for improving bacterial c비turability and narrowing the gap between microbial diversity in nature and cllltivable microorganisms.
URI
http://repository.kopri.re.kr/handle/201206/6423
DOI
http://dx.doi.org/10.1127/1863-9135/2013/0391
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