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Anticancer activities of ethanol extract from the Antarctic freshwater microalga, Botryidiopsidaceae sp.

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Title
Anticancer activities of ethanol extract from the Antarctic freshwater microalga, Botryidiopsidaceae sp.
Other Titles
남극담수미세조류, Botryidiopsidaceae sp,의 에탄올 추출물의 항암 활성
Authors
Suh, Sung-Suk
Kim, Sanghee
Kim, Il-Chan
Han, Se Jong
Youn, Ui Joung
Lee, Sung Gu
Hong, Ju Mi
Kim, Jung Eun
Kim, Sun-Mi
Subject
Integrative & Complementary Medicine
Keywords
Antarctic freshwater microalga; Botryidiopsidaceae sp.; anti-proliferation; anticancer activities
Issue Date
2017
Citation
Suh, Sung-Suk, et al. 2017. "Anticancer activities of ethanol extract from the Antarctic freshwater microalga, Botryidiopsidaceae sp.". BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, 17(1): 509-517.
Abstract
Background: Cancer is a leading cause of human death around the world and occurs through the highly complex coordination of multiple cellular pathways. Recent studies have revealed that microalgal extracts exhibit considerable pharmaceutical activities, including those against various cancer cells. Thus, microalgae are promising candidates as novel cancer therapeutic drugs. In this study, we evaluated the biological functions of the ethanolic extract of the Antarctic freshwater microalga, Botryidiopsidaceae sp., such as its antioxidant, anti-proliferative, apoptotic and anti-invasive properties. Methods: To estimate antioxidant capacity of ethanol extract of Botryidiopsidaceae sp. (ETBO), free radical 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays were used. The anti-proliferative activity of ETBO was assessed in several cancer cell lines (A375, Hs578T and HeLa) and non-tumorigenic keratinocyte cells (HaCaT), using MTT assay. In addition, Annexin V binding was performed to detect ETBO-induced apoptotic cells, and the expression levels of apoptosis-regulating proteins, caspase-3, p53, and Bcl-2, were determined by western blot. Boyden chamber assays were used to determine anti-migratory and anti-invasive properties of ETBO. Results: ETBO exhibited antioxidant activity and concentration-dependent anticancer activities, such as anti-proliferation and pro-apoptotic activities against cancer cells. Furthermore, the expression of the apoptosis-inducing proteins, p53 and caspase-3, significantly increased in response to ETBO, whereas the expression of the anti-apoptotic protein, Bcl-2, decreased. These data imply that ETBO induces apoptosis by caspase activation through the modulation of pro-apoptotic and anti-apoptotic gene, p53 and Bcl-2, respectively. In addition, ETBO significantly inhibited migration and invasion of cervical cancer cells in a concentration-dependent manner. Conclusion: In this study, ETBO exhibited considerable anticancer activities, such as inhibition of proliferation, invasion, and migration, as well as induction of apoptosis. These data suggest that ETBO is a promising therapeutic agent in cancer therapy and drug discovery.
URI
http://repository.kopri.re.kr/handle/201206/6456
DOI
http://dx.doi.org/10.1186/s12906-017-1991-x
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