KOPRI Repository

A Modified Cryopreservation Method of Psychrophilic Chlorophyta Pyramimonas sp. from Antarctica

Cited 1 time in scopus
Metadata Downloads
Title
A Modified Cryopreservation Method of Psychrophilic Chlorophyta Pyramimonas sp. from Antarctica
Other Titles
남극 호냉성 녹조 파이라미모나스의 동결보존 방법 개선연구
Authors
Hong, Sung Soo
Kang, Sung-Ho
Lee, Soo Young
Kim, Hak Jun
Kim, Young Nam
Subject
Oceanography
Keywords
Cryopreservation; Pyramimonas; antifreeze activity; microalgae; psychrophile
Issue Date
2011
Publisher
한국해양연구원
Citation
Hong, Sung Soo, et al. 2011. "A Modified Cryopreservation Method of Psychrophilic Chlorophyta Pyramimonas sp. from Antarctica". Ocean and Polar Research, 33(3): 303-308.
Abstract
Polar psychrophiles which thrive under extreme conditions such as cold temperature, high salinity, and high dose ultraviolet light, emerge as novel targets for biotechnology. To prevent genetic drift and the possibility of contamination by subculturing, cryopreservation was employed for two psychrophilic microalgae, Porosira sp. and Pyramimonas sp. (KOPRI AnM0046), which have antifreeze activities. Five cryoprotectants (dimethyl sulphoxide, ethylene glycol, glycerol, methanol and propylene glycol) showed toxicity at 20-30% (v/v). The optimal cryoprotectant concentration and equilibration time were less than 20% and 10 min, respectively. Cryopreservation was carried out in the presence of cryoprotectants either by direct freezing in liquid nitrogen (LN2) or controlled freezing using a controlled rate freezer followed by storage in the LN2 tank. As a result, Pyramimonas sp. (KOPRI AnM0046), a psychrophilic chlorophyta was revived. Cryopreserved Porosira sp. was not revived from either freezing protocols probably due to the silicic cell wall and its relatively large cell size. In the case of Pyramimonas sp. (KOPRI AnM0046), the controlled freezing method showed higher revival yield than the direct freezing method. by subculturing, cryopreservation was employed for two psychrophilic microalgae, Porosira sp. and Pyramimonas sp. (KOPRI AnM0046), which have antifreeze activities. Five cryoprotectants (dimethyl sulphoxide, ethylene glycol, glycerol, methanol and propylene glycol) showed toxicity at 20-30% (v/v). The optimal cryoprotectant concentration and equilibration time were less than 20% and 10 min, respectively. Cryopreservation was carried out in the presence of cryoprotectants either by direct freezing in liquid nitrogen (LN2) or controlled freezing using a controlled rate freezer followed by storage in the LN2 tank. As a result, Pyramimonas sp. (KOPRI AnM0046), a psychrophilic chlorophyta was revived. Cryopreserved Por
URI
http://repository.kopri.re.kr/handle/201206/6535
DOI
http://dx.doi.org/10.4217/OPR.2011.33.3.303
Files in This Item
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse