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Axenic purification and cultivation of an Arctic cyanobacterium, Nodularia spumigena KNUA005, with cold tolerance potential for sustainable production of algae-based biofuel

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Title
Axenic purification and cultivation of an Arctic cyanobacterium, Nodularia spumigena KNUA005, with cold tolerance potential for sustainable production of algae-based biofuel
Other Titles
조류 기반 바이오 연료의 지속가능한 생산을 위한 저온 저항 잠재력을 갖는 북극 시아노 박테리아 Nodularia spumigena KNUA005의 배양과 순수 정제
Authors
Hong, Ji Won
Choi, Han-Gu
Yoon, Ho-Sung
Kang, Sung-Ho
Subject
Life Sciences & Biomedicine - Other Topics
Keywords
Nodularia spumigena; biofuel; cold-tolerance; cyanobacterium
Issue Date
2010
Publisher
The Korean Society of Phycology
Citation
Hong, Ji Won, et al. 2010. "Axenic purification and cultivation of an Arctic cyanobacterium, Nodularia spumigena KNUA005, with cold tolerance potential for sustainable production of algae-based biofuel". Algae, 25: 99-104.
Abstract
A psychrotolerant cyanobacterium, Nodularia spumigena KNUA005, was isolated from a cyanobacterial bloom sample collected near Dasan Station in Ny-Alesund, Svalbard Islands during the Arctic summer season. To generate an axenic culture, the isolate was subjected to three purification step: centrifugation, antibiotic treatment and streaking. The broad antibacterial spectrum of imipenem killed a wide range of heterotrophic bacteria, while the cyanobacterium was capable of enduring both antibiotics, the remaining contaminants that survived after treatment with imipenem were eliminated by the application of an aminoglycoside antibiotic, kanamycin. Physical separation by centrifugation and streaking techniques also aided axenic culture production. According to the cold-tolerance test, this mat-forming cyanobacterium was able to proliferate at low temperatures ranging between 15 and 20℃ which indicates the presence of cold-tolerance related genes in N. spumigena KNUA005. This suggests the possibility of incorporating cold-resistance gene into indigenous cyanobacterial strains for the consistent production of algae-based biofuel during the low-temperature seasons. Therefore, it is needed to determine the cold-tolerance mechanisms in the Arctic cyanobacterium in the next research stage.
URI
http://repository.kopri.re.kr/handle/201206/6544
DOI
http://dx.doi.org/10.4490/algae.2010.25.2.099
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