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Determination of the Minimal Sequence Required for Antifreeze Activity of Type I Antifreeze Protein (AFP37)

Cited 2 time in wos
Cited 3 time in scopus

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dc.contributor.authorJung, Woongsic-
dc.contributor.authorPark, Kyoung Sun-
dc.contributor.authorShin, Song Yub-
dc.contributor.authorKim, Hak Jun-
dc.date.accessioned2018-03-20T14:00:04Z-
dc.date.available2018-03-20T14:00:04Z-
dc.date.issued2010-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/6546-
dc.description.abstractIn order to elucidate the minimal sequence required for antifreeze activity of AFP 37, we synthesized a series of N- and C-terminal truncated peptides of AFP 37 with the central two Thr residues (Thr-13 and Thr-24) (Table 1). These truncated peptides (AFP 34, AFP 32, AFP 30, AFP 28, AFP 26, AFP 24, AFP 22 and AFP 20) were synthesized by a stepwise deletion of one residue or two residues from N- and C-terminus of AFP 37. Table 2 summarizes the thermal hysteresis values obtained with a series of truncated peptides of AFP 37. AFP 37, AFP 34, AFP 32, AFP 30 and AFP 28 show a linear dependence of the antifreeze activity on the concentrations of the peptides. AFP 34, AFP 32, AFP 30 and AFP 28 preserved 99.1%, 62.6%,56.16% and 15.9%thermal hysteresis activity of the wild type AFP (AFP 37) at a peptide concentration of 40 mg/mL, respectively. However, a significant hysteresis was not observed with any of the AFP 26, AFP24, AFP22 and AFP 20 at 40 mg/mL (data not shown). These results suggested that the minimalsequence required for antifreeze activity is from residue 5 toresidue 32 of AFP 37 (AFP 28).-
dc.languageEnglish-
dc.publisher대한화학회-
dc.subjectChemistry-
dc.titleDetermination of the Minimal Sequence Required for Antifreeze Activity of Type I Antifreeze Protein (AFP37)-
dc.typeArticle-
dc.identifier.bibliographicCitationJung, Woongsic, et al. 2010. "Determination of the Minimal Sequence Required for Antifreeze Activity of Type I Antifreeze Protein (AFP37)". <em>BULLETIN OF THE KOREAN CHEMICAL SOCIETY</em>, 31(12): 3791-3793.-
dc.citation.titleBULLETIN OF THE KOREAN CHEMICAL SOCIETY-
dc.citation.volume31-
dc.citation.number12-
dc.identifier.doi10.5012/bkcs.2010.31.12.3791-
dc.citation.startPage3791-
dc.citation.endPage3793-
dc.description.articleClassificationSCI-
dc.description.jcrRateJCR 2008:49.60629921259843-
dc.subject.keywordAntifreeze protein-
dc.subject.keywordThermal hysteresis-
dc.subject.keywordType I antifreeze protein(AFP-
dc.identifier.localId2010-0190-
dc.identifier.scopusid2-s2.0-78650383605-
dc.identifier.wosid000286150900057-
Appears in Collections  
2008-2010, Development of Longer preservation of Blood Using Antifreeze Molecules Derived from Polar Organisms (08-10) / Kim, Hak Jun (PG08040, PE09070, PE10070)
2010-2010, Developing a cryoprotectant candidate derived from antifreeze protein for the cryopreservation of valuable bioresources (10-10) / Kim, Hak Jun (PG10010)
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