KOPRI Repository

Pilot-scale production of the antifreeze protein from Antarctic bacterium Flavobacterium frigoris PS1 by recombinant Escherichia coli with a cold shock induction system

Cited 0 time in scopus
Metadata Downloads
Title
Pilot-scale production of the antifreeze protein from Antarctic bacterium Flavobacterium frigoris PS1 by recombinant Escherichia coli with a cold shock induction system
Other Titles
저온 유도 시스템을 가진 재조합 대장균을 이용한 남극 세균 Flavobacterium frigoris PS1 유래 결빙방지단백질의 Pilot-scale 생산
Authors
Kim, Eun Jae
Han, Se Jong
Lee, Sung Gu
Lee, Jun Hyuck
Subject
Engineering
Keywords
Antifreeze protein; Cold shock induction; Flavobacterium frigoris; Pilot scale fermentation
Issue Date
2015
Citation
Kim, Eun Jae, et al. 2015. "Pilot-scale production of the antifreeze protein from Antarctic bacterium Flavobacterium frigoris PS1 by recombinant Escherichia coli with a cold shock induction system". KSBB Journal, 30(6): 345-349.
Abstract
Antifreeze proteins (AFP) inhibit growth and recrystallization of ice, and permit organisms to survive in cold environments. The AFP from an Antarctic bacterium, Flavobacterium frigoris PS1, FfIBP (Flavobacterium frigoris ice-binding protein), was produced in E. coli using a cold shock induction system. The culture temperature was shifted from 37°C to 15°C and a 20 L culture scale was used. The final weights of dried cell and FfIBP were estimated to be 126 g and 8.4 g, respectively. The thermal hysteresis (TH) activity (1.53°C) of the produced FfIBP was 3.6-fold higher than that of the LeIBP (Leucosporidium ice-binding protein) produced in Picha. The current study demonstrates that large-scale production of FfIBP was successful and the result could be extended to further application studies using recombinant AFPs.
URI
http://repository.kopri.re.kr/handle/201206/6580
DOI
http://dx.doi.org/10.7841/ksbbj.2015.30.6.345
Files in This Item
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse