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Pilot-scale production of recombinant LeIBP from Arctic yeast Glaciozyma sp. by Escherichia coli

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Title
Pilot-scale production of recombinant LeIBP from Arctic yeast Glaciozyma sp. by Escherichia coli
Other Titles
대장균을 이용한 북극 효모 Glaciozyma sp. 유래 재조합 결빙방지단백질 LeIBP의 pilot-scale 생산
Authors
Kim, Eun Jae
Han, Se Jong
Lee, Sung Gu
Lee, Jun Hyuck
Keywords
Antifreeze protein; Arctic; Glaciozyma sp.; LeIBP; Pilot-scale fermentation; Ergonomics
Issue Date
2017
Citation
Kim, Eun Jae, et al. 2017. "Pilot-scale production of recombinant LeIBP from Arctic yeast Glaciozyma sp. by Escherichia coli". KSBB Journal, 32(4): 300-305.
Abstract
Antifreeze proteins (AFPs) have a unique feature to bind to ice nuclei, and are referred to ice-binding protein (IBP). The AFP, expressed in the cells of some polar organisms, controls cell damage in subzero temperature environments by inhibiting the ice growth and recrystallization. In this study, recombinant LeIBP (Leucosporidium ice-binding protein) from the arctic yeast Glaciozyma sp. (former known as Leucosporidium sp.) was produced in E. coli using a cold shock induction system. The final cell density and concentration of the purified LeIBP were measured to be 8.2 g/L and 1.1 g/L, respectively. The thermal hysteresis (TH) activity of LeIBP was 0.39 ± 0.02°C at 13.25 mg/ml, which means that the scale-up was successfully performed for the production of recombinant LeIBP in heterologous bacterial expression system.
URI
http://repository.kopri.re.kr/handle/201206/6581
DOI
http://dx.doi.org/10.7841/ksbbj.2017.32.4.300
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