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Pilot-scale production of recombinant LeIBP from Arctic yeast Glaciozyma sp. by Escherichia coli

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dc.contributor.authorKim, Eun Jae-
dc.contributor.authorHan, Se Jong-
dc.contributor.authorLee, Sung Gu-
dc.contributor.authorLee, Jun Hyuck-
dc.coverage.spatialArctic-
dc.date.accessioned2018-03-20T14:02:33Z-
dc.date.available2018-03-20T14:02:33Z-
dc.date.issued2017-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/6581-
dc.description.abstractAntifreeze proteins (AFPs) have a unique feature to bind to ice nuclei, and are referred to ice-binding protein (IBP). The AFP, expressed in the cells of some polar organisms, controls cell damage in subzero temperature environments by inhibiting the ice growth and recrystallization. In this study, recombinant LeIBP (Leucosporidium ice-binding protein) from the arctic yeast Glaciozyma sp. (former known as Leucosporidium sp.) was produced in E. coli using a cold shock induction system. The final cell density and concentration of the purified LeIBP were measured to be 8.2 g/L and 1.1 g/L, respectively. The thermal hysteresis (TH) activity of LeIBP was 0.39 ± 0.02°C at 13.25 mg/ml, which means that the scale-up was successfully performed for the production of recombinant LeIBP in heterologous bacterial expression system.-
dc.languageKorean-
dc.titlePilot-scale production of recombinant LeIBP from Arctic yeast Glaciozyma sp. by Escherichia coli-
dc.title.alternative대장균을 이용한 북극 효모 Glaciozyma sp. 유래 재조합 결빙방지단백질 LeIBP의 pilot-scale 생산-
dc.typeArticle-
dc.identifier.bibliographicCitationKim, Eun Jae, et al. 2017. "Pilot-scale production of recombinant LeIBP from Arctic yeast Glaciozyma sp. by Escherichia coli". <em>KSBB Journal</em>, 32(4): 300-305.-
dc.citation.titleKSBB Journal-
dc.citation.volume32-
dc.citation.number4-
dc.identifier.doi10.7841/ksbbj.2017.32.4.300-
dc.citation.startPage300-
dc.citation.endPage305-
dc.description.articleClassificationKCI등재-
dc.description.jcrRateJCR 2015:0-
dc.subject.keywordAntifreeze protein-
dc.subject.keywordArctic-
dc.subject.keywordGlaciozyma sp.-
dc.subject.keywordLeIBP-
dc.subject.keywordPilot-scale fermentation-
dc.subject.keywordErgonomics-
dc.identifier.localId2017-0213-
Appears in Collections  
2016-2018, Cryo-Biotech (16-18) / Yim, Joung Han; Han, Se Jong (PE16190; PE17100; PE18100)
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