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Characterization of a Chitinase Gene and Screening of Cold ActiveChitinase from Polar Microorganisms

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Title
Characterization of a Chitinase Gene and Screening of Cold ActiveChitinase from Polar Microorganisms
Other Titles
극지유래 저온활성 chitinase 생산균주의 스크리닝과 chitinase 유전자 클로닝
Authors
Kim, Jung Eun
Park, Yoo Kyung
Kim, Il-Chan
Yim, Joung Han
Park, Mira
Kim, Dockyu
Park, Ha Ju
Kim, Ji Hyun
Lee, Hyoungseok
Subject
Life Sciences & Biomedicine - Other Topics
Keywords
chitinase; cold-active chitinase; iPCR; polar microorganisms; screening
Issue Date
2012
Publisher
The Microbiological Society of Korea
Citation
Kim, Jung Eun, et al. 2012. "Characterization of a Chitinase Gene and Screening of Cold ActiveChitinase from Polar Microorganisms". Korean Journal of Microbiology, 48(4): 293-297.
Abstract
Of the 169 strains of microorganisms stored in Polar and Alpine Microbial Collection of Korea Polar Research Institute, 27 strains were selected for their chitinase activity on ZoBell plates supplemented with 0.4% colloidal chitin. Among them, PAMC strain have shown the highest chitinolytic enzyme activity toward pNP-(GlcNAc)1 at low temperature and the highest growth rate at 4°C. We cloned a full-length chitinase gene of 2,857 bp which contains an open reading frame of 2,169 bp encoding 872-amino acid polypeptide. Recombinant chitinase protein was expressed in E. coli and its molecular weight was confirmed 96 kDa. In this paper, we suggest the potential use of cold-active chitinase from polar microorganisms in the field of biotechnology.g them, PAMC strain have shown the highest chitinolytic enzyme activity toward pNP-(GlcNAc)1 at low temperature and the highest growth rate at 4°C. We cloned a full-length chitinase gene of 2,857 bp which contains an open reading frame of 2,169 bp encoding 872-amino acid polypeptide. Recombinant chitinase protein was expressed in E. coli and its molecular weight was confirmed 96 kDa. In this paper, we suggest the potential use of cold-active chitinase from polar microorganisms in the field of biotechnology.
URI
http://repository.kopri.re.kr/handle/201206/6582
DOI
http://dx.doi.org/10.7845/kjm.2012.030
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