KOPRI Repository

Annotation?and?expression?profile?analysis?of?cIDNAs?from?the?Antarctic?diatom?Chaetocerosneogracile

Cited 0 time in scopus
Metadata Downloads
Title
Annotation?and?expression?profile?analysis?of?cIDNAs?from?the?Antarctic?diatom?Chaetocerosneogracile
Other Titles
남극 규조류 Chaetoceros neogracile의 cDNAs의 Annotation and expression profile 분석
Authors
정경서
이철균
Jin, EonSeon
Kang, Sung-Ho
Subject
Biotechnology & Applied Microbiology; Microbiology
Keywords
Chaetoceros neogracile; ?antarctic diatom; ?ESTs; ?cold adaptation
Issue Date
2007
Citation
정경서, et al. 2007. "Annotation?and?expression?profile?analysis?of?cIDNAs?from?the?Antarctic?diatom?Chaetocerosneogracile". JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 17(8): 1330-1337.
Abstract
To better understand the gene expression of the cold-adapted polar diatom, we conducted a survey of the Chaetoceros neogracile transcriptome by cDNA sequencing and expression of interested cDNAs from the Antarctic diatom. A non-normalized cDNA library was constructed from the C. neogracile, and a total of 2,500 cDNAs were sequenced to generate 1,881 high-quality expressed sequence tags (ESTs) (accession numbers EL620615-EL622495). Based on their clustering, we identified 154 unique clusters comprising 342 ESTs. The remaining 1,540 ESTs did not cluster. The number of unique genes identified in the data set is thus estimated to be 1,694. Taking advantage of various tools and databases, putative functions were assigned to 939 (55.4%) of these genes. Of the remaining 540 (31.9%) unknown sequences, 215 (12.7%) appeared to be C. neogracile-specific since they lacked any significant sequence similarity to any sequence available in the public databases. C. neogracile consisted of a relatively high percentage of genes involved in metabolism, genetic information processing, cellular processes, defense or stress resistance, photosynthesis, structure, and signal transduction. From the ESTs, the expression of these putative C. neogracile genes was investigated: fucoxanthin chlorophyll (chl) a,c-binding protein (FCP), ascorbate peroxidase (ASP), and heat-shock protein 90 (HSP90). The abundance of ASP and HSP90 changed substantially in response to different culture conditions, indicating the possible regulation of these genes in C. neogracile.
URI
http://repository.kopri.re.kr/handle/201206/6602
Files in This Item
There are no files associated with this item.
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse