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Expressed sequence tag analysis of Antarctic hairgrass Deschampsia antarctica from King George Island, Antarctica

Cited 16 time in scopus
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Title
Expressed sequence tag analysis of Antarctic hairgrass Deschampsia antarctica from King George Island, Antarctica
Other Titles
남극 킹죠지섬에서 분리된 좀새풀의 발현유전자 분석
Authors
Lee, Hyoungseok
Lee, Yoo Kyung
Yim, Joung Han
Lee, Hong Kum
Kim, Il-Chan
Cho, Hyun Hee
Subject
Environmental Sciences & Ecology; Marine & Freshwater Biology
Keywords
Abiotic Stress; Antarctic; Deschampsia antarcitca; Expressed Sequence Tags (ESTs); Quantitative Real Time Reverse
Issue Date
2008
Citation
Lee, Hyoungseok, et al. 2008. "Expressed sequence tag analysis of Antarctic hairgrass Deschampsia antarctica from King George Island, Antarctica". MOLECULES AND CELLS, 25: 258-264.
Abstract
Deschampsia antarctica is the only monocot that thrives in the tough conditions of the Antarctic region. It is an invaluable resource for the identification of genes associated with tolerance to various environmental pressures. In order to identify genes that are differentially regulated between greenhouse-grown and Antarctic field-grown plants, we initiated a detailed gene expression analysis. Antarctic plants were collected and greenhouse plants served as controls. Two different cDNA libraries were constructed with these plants. A total of 2,112 cDNA clones was sequenced and grouped into 1,199 unigene clusters consisting of 243 consensus and 956 singleton sequences. Using similarity searches against several public databases, we constructed a functional classification of the ESTs into categories such as genes related to responses to stimuli, as well as photosynthesis and metabolism. Real-time PCR analysis of various stress responsive genes revealed different patterns of regulation in the different environments, suggesting that these genes are involved in responses to specific environmental factors.
URI
http://repository.kopri.re.kr/handle/201206/6606
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