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Enhanced Production ofEndochitinase from An Antarctic Bacterium,Sanguibacter antarcticus PAMC21702, in Pichia pastoris

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dc.contributor.authorHan, Se Jong-
dc.contributor.authorYim, Joung Han-
dc.contributor.authorKoh, Hye Yeon-
dc.contributor.authorPark, Heeyong-
dc.contributor.authorLee, Sung Gu-
dc.coverage.spatialAntarctica-
dc.date.accessioned2018-03-20T14:04:25Z-
dc.date.available2018-03-20T14:04:25Z-
dc.date.issued2014-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/6612-
dc.description.abstractThe recombinant endochitinase (rChi21702) from a psychrophilic Antarctic bacterium, Sanguibacter antarcticus (KCTC 13143, PAMC 21702), has been produced using Pichia pastoris expression system. In this study, the optimum fermentation temperature (20°C) and growth media pH (6) for Chi21702 production was determined using a 2-stage fed-batch culture system. The maximal enzyme activity (178 U/l) of rChi21702 obtained using a glycerol-methanol fed-batch culture system was approximately 6-fold higher than that of unoptimized conditions. In addition, the rChi21702 exhibited cold-active endochitinase activity and higher thermal stability than that of the wild-type Chi21702. This work allows for large-scale production of rChi21702, which could be extended to further application studies using recombinant chtinases.-
dc.languageEnglish-
dc.titleEnhanced Production ofEndochitinase from An Antarctic Bacterium,Sanguibacter antarcticus PAMC21702, in Pichia pastoris-
dc.title.alternative피키아 패스토리스에서 남극세균 상귀박터 안탁티쿠스 PAMC 21702 유래 키틴분해효소의 생산성 증대-
dc.typeArticle-
dc.identifier.bibliographicCitationHan, Se Jong, et al. 2014. "Enhanced Production ofEndochitinase from An Antarctic Bacterium,Sanguibacter antarcticus PAMC21702, in Pichia pastoris". <em>JOURNAL OF PURE AND APPLIED MICROBIOLOGY</em>, 8(5): 3531-3536.-
dc.citation.titleJOURNAL OF PURE AND APPLIED MICROBIOLOGY-
dc.citation.volume8-
dc.citation.number5-
dc.citation.startPage3531-
dc.citation.endPage3536-
dc.description.articleClassificationSCIE-
dc.description.jcrRateJCR 2012:97.5-
dc.subject.keywordAntarctica-
dc.subject.keywordCold-active-
dc.subject.keywordEndochitinases-
dc.subject.keywordFed-batch-
dc.subject.keywordPichia pastoris-
dc.identifier.localId2014-0014-
dc.identifier.scopusid2-s2.0-84923855349-
Appears in Collections  
2011-2013, Utilization of novel metabolites from polar organisms (11-13) / Yim, Joung Han (PE11060, PE12040, PE13040)
2009-2010, 극지 미생물 유래 재조합 단백질 생산 시스템 개발 (09-10) / Han, Se Jong (PK09040)
2013-2015, Production of bioenergy using cold resistance polar organisms (13-15) / Han, Se Jong (PE13150; PE14140; PE15140)
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