Physicocehmical and rheological properties of a novel emulsifier, EPS-R, produced by the marine bacterium Hahella chejuensis
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- Physicocehmical and rheological properties of a novel emulsifier, EPS-R, produced by the marine bacterium Hahella chejuensis
- Other Titles
- 하헬라 JeJu엔시스가 생산하는 신규 생물유화제 EPS=R의 물리화학적 특성 및 물성
- Yim, Joung Han
Kim, Sung Jin
An, Se Hoon
Lee, Hong Kum
- Hahella chejuensis; exopolysaccharide; marine bacterium; rheological properties
- Issue Date
- Yim, Joung Han, et al. 2004. "Physicocehmical and rheological properties of a novel emulsifier, EPS-R, produced by the marine bacterium Hahella chejuensis". Biotechnology and Bioprocess Engineering, 9(5): 1.
- The rheological properties of an exopolysaccharide, EPS-R, produced by the marine bacterium Hahella chejuensis strain 96CJ10356 were investigated. The E24 of 0.5% EPS-R was 89.2%, which was higher than that observed in commercial polysaccharides such as xanthan gum (67.8%), gellan gum (2.01%) or sodium alginate (1.02%). Glucose and galactose are the main sugars in EPS-R, with a molar ratio of ~1:6.8, xylose and ribose are minor sugar components. The average molecular mass, as determined by gel filtration chromatography, was 2.2 x 103 KDa. The intrinsic viscosities of EPS-R were calculated to be 16.5 and 15.9 dl/g using the Huggins and Kraemer equations, respectively, with a 2.3 dl/g overlap. In terms of rigidity, the conformation of EPS-R was similar to that of caboxymethyl cellulose (5.0 x 10-2). The rheological behavior of EPS？R dispersion indicated that the formation of a structure intermediate between that of a random-coil polysaccharide and a weak gel. The aqueous dispersion of EPS-R at concentrations ranging from 0.25% to 1.0% (w/w) showed a marked shear-thinning property in accordance with Power-law behavior. In aqueous dispersions of 1.0% EPS-R, the consistency index (K) and flow behavior index (n) were 1,410 and 0.73, respectively. EPS-R was stable to pH and salts.
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