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An Efficient Method to Prepare PCR Cloning Vectors

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Title
An Efficient Method to Prepare PCR Cloning Vectors
Other Titles
PCR 클로닝 벡서를 만들기 위하 효율적인 방법
Authors
Hong, Soon Gyu
Lee, Hong Kum
BM Pryor
최지영
Subject
Life Sciences & Biomedicine - Other Topics
Keywords
PCR cloning vector; XcmI
Issue Date
2009
Citation
Hong, Soon Gyu, et al. 2009. "An Efficient Method to Prepare PCR Cloning Vectors". The Korean Society of Mycology, 37(3): 240-242.
Abstract
An improved procedure for preparing PCR cloning vectors was developed. This procedure includes the incorporation ofadapters to create XcmI restriction enzyme sites in pBluescript II SK(+) vectors, digestion with XcmI followed by furtherdigestion of the small fragment produced by XcmI digestion with additional enzymes, and purification with PCR purificationkits. Using this procedure, PCR cloning vectors with high ligation efficiencies and low blue or false-positive colonies wereobtained.
URI
http://repository.kopri.re.kr/handle/201206/6662
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