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Screening for Cold-Active Protease-Producing Bacteria from the Culture Collection of Polar Microorganisms and Characterization of Proteolytic Activities

Cited 5 time in scopus
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Title
Screening for Cold-Active Protease-Producing Bacteria from the Culture Collection of Polar Microorganisms and Characterization of Proteolytic Activities
Other Titles
남북극 유래 저온성 박테리아 culture collection에서 저온활성 프로테아제 생산균주의 스크리닝과 효소 특성
Authors
Park, Ha Ju
Kim, Dockyu
Lee, Yung Mi
Yim, Joung Han
Lee, Hong Kum
Hong, Soon Gyu
Subject
Life Sciences & Biomedicine - Other Topics
Keywords
Antarctic; Arctic; cold-active; culture collection; protease
Issue Date
2010
Citation
Park, Ha Ju, et al. 2010. "Screening for Cold-Active Protease-Producing Bacteria from the Culture Collection of Polar Microorganisms and Characterization of Proteolytic Activities". The Microbiological Society Of Korea, 46(1): 73-79.
Abstract
The Korea Polar Research Institute (KOPRI) has assembled a culture collection of cold-adapted bacterial strains from both the Arctic and Antarctic. To identify excellent protease-producers among the proteolytic bacterial collection (874 strains), 78 strains were selected in advance according to their relative activities and were subsequently re-examined for their extracellular protease activity on 0.1×ZoBell plates supplemented with 1% skim milk at various temperatures. This rapid and direct screening method permitted the selection of a small group of 15 cold-adapted bacterial strains,belonging to either the genus Pseudoalteromonas (13 strains) or Flavobacterium (2 strains), that showed proteolytic activities at temperatures ranging between 5-15?°C. The cold-active proteases from these strains were classified into four categories (serine protease, aspartic protease, cysteine protease, and metalloprotease) according to the extent of enzymatic inhibition by a class-specific protease inhibitor.Since highly active and/or cold-adapted proteases have the potential for industrial or commercial enzyme development, the protease-producing bacteria selected in this work will be studied as a valuable natural source of new proteases. Our results also highlight the relevance of the Antarctic for the isolation of protease-producing bacteria active at low temperatures.
URI
http://repository.kopri.re.kr/handle/201206/6666
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