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Fed-batch production of ice-binding protein of Flavobacterium frigoris PS1 by recombinant Pichia pastoris

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Title
Fed-batch production of ice-binding protein of Flavobacterium frigoris PS1 by recombinant Pichia pastoris
Other Titles
재조합 Pichia pastoris에 의한 Flavobacterium frigoris PS1 얼음결합단백질의 유가식 생산
Authors
Kim, Eun Jae
Han, Se Jong
Kim, Hak Jun
Lee, Sung Gu
Lee, Jun Hyuck
Do, Hackwon
Keywords
Antifreeze protein; Fed-batch fermentation; Ice-binding protein
Issue Date
2015
Citation
Kim, Eun Jae, et al. 2015. Fed-batch production of ice-binding protein of Flavobacterium frigoris PS1 by recombinant Pichia pastoris. 2015 The Korea Society of Marine and Fisheries Life Science Spring Meeting. Pukyong National University. 2015.04.17~2015.04.17.
Abstract
Ice-binding proteins (IBPs) inhibit ice growth to permit the survival of polar organisms in the cold environments. The recombinant IBP from an Antarctic bacterium, Flavobacterium frigoris PS1, FfIBP (Flavobacterium frigoris ice-binding protein), was produced using Pichia pastoris expression system. The optimum fermentation temperature and pH for fed-batch production of FfIBP were 30℃ and 5, respectively. The maximal cell density and purified FfIBP were 112 g/L and 70 mg/L, respectively. The thermal hysteresis (TH) activity (0.85℃) of FfIBP obtained using a glycerol-methanol fed-batch culture system was 2-fold higher than that of the LeIBP (Leucosporidium ice-binding protein). This work allows for large-scale production of FfIBP, which could be extended to further application studies using recombinant IBPs.
URI
http://repository.kopri.re.kr/handle/201206/7104
Conference Name
2015 The Korea Society of Marine and Fisheries Life Science Spring Meeting
Conference Place
Pukyong National University
Conference Date
2015.04.17~2015.04.17
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