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MARINE RNA VIRUS COMMUNITIES OF OCEANIC SEAWATERS IN THE VICINITY OF THE ANTARCTIC PENINSULA

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Title
MARINE RNA VIRUS COMMUNITIES OF OCEANIC SEAWATERS IN THE VICINITY OF THE ANTARCTIC PENINSULA
Other Titles
남극반도의 해수 marine RNA 바이러스 군집연구
Authors
Hwang, Chung Yeon
Grieg F. Steward
Jaclyn A. Mueller
Issue Date
2015
Citation
Hwang, Chung Yeon, Grieg F. Steward, Jaclyn A. Mueller. 2015. MARINE RNA VIRUS COMMUNITIES OF OCEANIC SEAWATERS IN THE VICINITY OF THE ANTARCTIC PENINSULA. 6th International Conference on Polar and Alpine Microbiology. University ofSouth Bohemia in ceske Budejovice. 2015.12.06~2015.12.10.
Abstract
Viruses play an important role in the biogeochemical cycle and the gene flux in marine plankton ecosystems. Multiple lines of evidence that RNA viruses comprise a significant fraction of virioplakton are emerging. However, no data on oceanic RNA viromes in the Antarctic seas are available. During the IBRV Araon cruise in 2013, each 50 L of surface seawaters at 4 offshore stations was collected and filtered to concentrate viruses by the FeCl3 flocculation method. Marine viruses were recovered and purified by the CsCl gradient ultracentrifugation. Nucleic acids of viral fractions were extracted and subsequently DNAs were removed by adding DNase. Metagenome libraries were generated from RNAs using the random priming-mediated sequence-independent single-primer amplification. Size ranges of 300-600 bp in the libraries were selected and sequenced by 454 pyrosequencing with the GS-FLX Titanium chemistry, yielding 333 Mb with 764,764 reads as raw data in total. In the presentation, results and its implications will be discussed.
URI
http://repository.kopri.re.kr/handle/201206/7130
Conference Name
6th International Conference on Polar and Alpine Microbiology
Conference Place
University ofSouth Bohemia in ceske Budejovice
Conference Date
2015.12.06~2015.12.10
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