KOPRI Repository

HIGH OCCURRENCE OF THERMOPHILIC BACTERIA ISOLATED FROM HYDROTHERMAL VENT PLUMES IN AUSTRALIAN-ANTARCTIC RIDGE, SOUTHERN OCEAN

Metadata Downloads
Title
HIGH OCCURRENCE OF THERMOPHILIC BACTERIA ISOLATED FROM HYDROTHERMAL VENT PLUMES IN AUSTRALIAN-ANTARCTIC RIDGE, SOUTHERN OCEAN
Other Titles
남극 AUSTRALIAN-ANTARCTIC RIDGE 열수에서 분리된 THERMOPHILIC BACTERIA
Authors
Yoon, Soo Jung
Lee, Hong Kum
Park, Sung Hyun
Rhee, Tae Siek
Hahm, Doshik
Hong, Soon Gyu
Lee, Yung Mi
Lee, Inae
Hwang, Chung Yeon
Issue Date
2015
Citation
Yoon, Soo Jung, et al. 2015. HIGH OCCURRENCE OF THERMOPHILIC BACTERIA ISOLATED FROM HYDROTHERMAL VENT PLUMES IN AUSTRALIAN-ANTARCTIC RIDGE, SOUTHERN OCEAN. 6th International Conference on Polar and Alpine Microbiology. University ofSouth Bohemia in ?eske Bud?jovice. 2015.09.06~2015.09.10.
Abstract
INTRODUCTION The intermediate spreading Australian-Antarctic Ridge (AAR) in the Southern Ocean is one of the largest unexplored regions of the global mid-ocean ridge systems because of limited accessibility due to harsh environment. Although activities of hydrothermal vents are supposed to be present in AAR, microbiological data are not available on cultivatable bacteria associated with those environments. METHODS AND MATERIALS During the IBRV Araon cruise in 2013, 14 samples of vent plumes were collected with Niskin bottles by CTD (conductivity, temperature and depth with potential reduction and turbidity sensors integrated) toyo-ing. Among the colonies grown on marine agar plates at 20°C from the vent samples, 139 colonies were randomly selected and purified for further investigations, determination of maximum temperature for growth and identification by 16S rRNA gene sequencing. RESULTS 52 out of 139 (ca. 37%) strains were able to grow at 45°C, which are referred as thermophlic bacteria here, while the others were mesophiles. Among the isolated thermophlic bacteria, 42 strains were affiliated with Firmicutes and 9 strains with Gamma-proteobacteria based on 16S rRNA gene sequences. Mesophilic bacteria comprised of Actinobacteria (7 strains) and the above-mentioned groups (i.e. 17 and 64 strains for Firmicutes and Gammaproteobacteria, respectively). Notably, two Bacillus lichneformis strains represented fast growing at the high temperature of 60°C, which are the first strains originated from deep-sea hydrothermal vent environments DISCUSSION As expected, thermophilic bacteria were abundant in the hydrothermal vent plumes. Gram-positive bacteria, Firmicutes, were dominant in the thermophilic bacteria in the study area, while Proteobacteria are often dominant in hydrothermal vents in other areas. This discrepancy might be attributed to different sources of vent fluids or different types of vent in respect to temperature and chemical compositions.
URI
http://repository.kopri.re.kr/handle/201206/7133
Conference Name
6th International Conference on Polar and Alpine Microbiology
Conference Place
University ofSouth Bohemia in ?eske Bud?jovice
Conference Date
2015.09.06~2015.09.10
Files in This Item
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse