KOPRI Repository

Expression profile analysis of antioxidative stress and developmental pathway genes in the manganese-exposed intertidal copepod Tigriopus japonicus with 6K oligochip

Cited 0 time in scopus
Metadata Downloads
Title
Expression profile analysis of antioxidative stress and developmental pathway genes in the manganese-exposed intertidal copepod Tigriopus japonicus with 6K oligochip
Other Titles
Manganese에 노출된 Tigriopus japonicus에서 6K oligochip을 이용한 항산화와 발생 경로에 관련된 유전자들의 발현 양상 분석
Authors
Kim, Bo-Mi
Jae-Seong Lee
Lee, Jae-Seong
Choi, Ik-Young
Kim, Il-Chan
Ki, Jang-Seu
Lee, Kyun-Woo
Choi, Beom-Soon
Keywords
Gene expression; Microarray; Mn; Real-time RT-PCR; Tigriopus japonicus
Issue Date
2013
Publisher
Elsevier
Citation
Kim, Bo-Mi, et al. 2013. "Expression profile analysis of antioxidative stress and developmental pathway genes in the manganese-exposed intertidal copepod Tigriopus japonicus with 6K oligochip". CHEMOSPHERE, 92(9): 1214-1223.
Abstract
Manganese (Mn) provides one of aquatic pollutants in marine ecosystem. Here we used a 6K oligomicroarray to identify the effect of Mn on transcriptomes in the copepod Tigriopus japonicus. A total of 5594 spots were significantly modulated on a 6K oligomicroarray with hierarchical clustering after exposure to Mn over 24 h. Of them, 186 and 489 genes were significantly upregulated and downregulated, respectively. Particularly, several genes involved in stress, detoxification, and developmental functions were significantly modulated in T. japonicus exposed for 24 h. In detail, Mn exposure specifically up-regulated genes that were related to intracellular stress, antioxidant, and detoxification pathways such as cytochrome P450s (CYPs), glutathione S-transferases (GSTs), and heat shock proteins (hsps), while a majority of downregulated genes was associated with developmental pathways such as cuticle protein, ecdysone receptor, and vitellogenin. These results demonstrated that Mn exposure modulated gene expression in relation to intracellular stress, leading to developmental retardation in the intertidal copepod, T. japonicus, and provide a better understanding of mechanistic molecular studies of Mn-induced cellular damage. on a 6K oligomicroarray with hierarchical clustering after exposure to Mn over 24 h. Of them, 186 and 489 genes were significantly upregulated and downregulated, respectively. Particularly, several genes involved in stress, detoxification, and developmental functions were significantly modulated in T. japonicus exposed for 24 h. In detail, Mn exposure specifically up-regulated genes that were related to intracellular stress, antioxidant, and detoxification pathways such as cytochrome P450s (CYPs), glutathione S-transferases (GSTs), and heat shock proteins (hsps), while a majority of downregulated genes was associated with developmental pathways such as cuticle protein, ecdysone receptor, and vitellogenin. These results demonstrated that Mn exposure modulated gene expression in relation to intracellular stress, leading to developmental retardation in the intertidal copepod, T. japonicus, and provide a better understanding of mechanistic molecular studies of Mn-induced cellular damage.
URI
http://repository.kopri.re.kr/handle/201206/7246
DOI
http://dx.doi.org/10.1016/j.chemosphere.2013.04.047
Files in This Item
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse