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Enhancing Extracellular Lipolytic Enzyme Production In An Arctic Bacterium, Psychrobacter sp. ArcL13, By Using Statistical Optimization And Fed-Batch Fermentation

Cited 2 time in scopus
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Title
Enhancing Extracellular Lipolytic Enzyme Production In An Arctic Bacterium, Psychrobacter sp. ArcL13, By Using Statistical Optimization And Fed-Batch Fermentation
Other Titles
통계적 최적화 및 유가식 발효를 이용한 북극해 박테리아로부터 지질분해효소의 생산성 향상
Authors
Han, Se Jong
Yim, Joung Han
Kim, Han-Woo
Kim, Dockyu
Park, Ha Ju
Wi, Ah Ram
김성희
Keywords
Arctic bacterium; Fed-batch fermentation; Lipolytic activity; Psychrophile; Statistical optimization
Issue Date
2015
Citation
Han, Se Jong, et al. 2015. "Enhancing Extracellular Lipolytic Enzyme Production In An Arctic Bacterium, Psychrobacter sp. ArcL13, By Using Statistical Optimization And Fed-Batch Fermentation". PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 45(4): 348-364.
Abstract
A strain isolated from seawater samples in the Chuckchi Sea and exhibiting extracellular lipolytic activity was identified using 16S rRNA gene sequence analysis as Psychrobacter sp. ArcL13. The lipolytic enzyme exhibited cold-active properties and high hydrolytic activity toward p-nitrophenyl caprylate (C8), p-nitrophenyl decanoate (C10), and sunflower oil. Statistical optimization of the medium components was performed to enhance the production of cold-active extracellular lipolytic activity. Glucose, yeast extract (YE), and NaCl were selected as the main efficient nutrient source. Fed-batch fermentation using optimized medium with concentrated YE as the main feeding material showed a maximum lipolytic activity of 10.7 U/mL, which was a 21-fold increase in production over unoptimized flask culture conditions. The information obtained in the present study could prove applicable to the production of cold-active lipase on a large scale.
URI
http://repository.kopri.re.kr/handle/201206/7256
DOI
http://dx.doi.org/10.1080/10826068.2014.940964
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