KOPRI Repository

Development of microalga Scenedesmus dimorphus mutant with higher lipid content by radiation breeding

Cited 9 time in scopus
Metadata Downloads
Title
Development of microalga Scenedesmus dimorphus mutant with higher lipid content by radiation breeding
Other Titles
방사선 육종에 의한 고농도 지질함유 Scenedesmus dimorphus 돌연변이 미세조류의 개발
Authors
Jong-il Choi
Pyung Cheon Lee
Han, Se Jong
Lee, Sung Gu
Park, Hyun
Minho Joe
Minchul Yoon
Keywords
2-Dimensional electrophoresis; Lipid content; Radiation breeding; Scenedesmus dimorphus
Issue Date
2014
Citation
Jong-il Choi, et al. 2014. "Development of microalga Scenedesmus dimorphus mutant with higher lipid content by radiation breeding". BIOPROCESS AND BIOSYSTEMS ENGINEERING, 37(12): 2437-2444.
Abstract
In this study, a high lipid-accumulating mutant strain of the microalgae Scenedesmus dimorphus was developed via radiation breeding. To induce mutant strain, S. dimorphus was gamma-irradiated at doses from 100 to 800 Gy, and then a mutant (Sd-Pm210) with 25 % increased lipid content was selected using Nile red staining methodology. Sd-Pm210 showed morphological changes and had higher growth rate compared to the wild type. From random amplified polymorphic DNA analysis, partial genetic modifications were also observed in Sd-Pm210. In comparisons of lipid content between wild type and Sd-Pm210 using thin-layer chromatography, the content of triacylglycerol was markedly higher in the Sd-Pm210 strain. The total peak area of fatty acid methyl ester was shown to have about 1.4-fold increase in Sd-Pm210, and major fatty acids were identified as palmitic acid, oleic acid, linoleic acid, and linolenic acid. To define the metabolic changes in the mutant strain, 2-dimensional electrophoresis was conducted. Several proteins related to lipid synthesis and energy metabolisms were overexpressed in the mutant strain. These results showed that radiation breeding can be utilized for the development of efficient microalgae strains for biofuel production.
URI
http://repository.kopri.re.kr/handle/201206/7270
DOI
http://dx.doi.org/10.1007/s00449-014-1220-7
Files in This Item
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse